Macrophage phenotypes and Gas6/Axl signaling in apical lesions

Szu Yu Chen, Chi Fu Chiang, Kou Chou Chiu, Chao Wen Cheng, Shih Ming Huang, Pei Hsuan Chen, Ching Yang Chen, Yi Shing Shieh

Research output: Contribution to journalArticle

Abstract

Background/purpose: Macrophages participate in the periapical inflammation with pro-inflammatory M1 cells and anti-inflammatory M2 cells. Gas6/Axl signal is the responsible pathway for the activation of M1 and polarization of M2. The aim of this study was to compare the number of CD16 + M1 cells, CD206 + M2 cells, and Gas6/Axl expression between apical granulomas and radicular cysts. Materials and methods: Twenty-four cases of granuloma and twenty of cysts were submitted to immunohistochemistry using anti-CD16 and anti-CD206 antibodies for determining M1 and M2 macrophages and investigating the cells with positive Gas6 and Axl expression. Results: There were more numerous of M1 macrophages in radicular cysts (175.9 ± 87.7) compared to apical granuloma (116.6 ± 55.8), and M2 macrophages was higher in cysts (204.0 ± 97.6) than granuloma (152.9 ± 64.6). The level of Gas6/Axl expression were similar. There was a significant different in M1 macrophage (P = 0.014) between two diagnosis. In patients with or without root resorption, the number of M1 were 194.6 ± 57.2 compared with 139.1 ± 79.6. The number of M2 were 241.7 ± 81.4 and 164.6 ± 77.1. The expression of Axl was stronger in root resorption patients (191.1 ± 43.6), but the tendency in Gas6 expression was similar. Significant differences were noted in high M2 infiltration and Axl positive lesions. Conclusion: It appears that macrophages associated with significantly higher numbers in radicular cysts than apical granuloma. Meanwhile, macrophages and Axl receptor was intensively expressed in patients with root resorption, related to severe inflammation.

Original languageEnglish
JournalJournal of Dental Sciences
DOIs
Publication statusPublished - Jan 1 2019

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Macrophages
Granuloma
Phenotype
Radicular Cyst
Root Resorption
Cysts
Inflammation
Anti-Idiotypic Antibodies
Anti-Inflammatory Agents
Immunohistochemistry

Keywords

  • Apical granuloma
  • Axl
  • Gas6
  • Macrophage
  • Radicular cyst

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Chen, S. Y., Chiang, C. F., Chiu, K. C., Cheng, C. W., Huang, S. M., Chen, P. H., ... Shieh, Y. S. (2019). Macrophage phenotypes and Gas6/Axl signaling in apical lesions. Journal of Dental Sciences. https://doi.org/10.1016/j.jds.2018.12.002

Macrophage phenotypes and Gas6/Axl signaling in apical lesions. / Chen, Szu Yu; Chiang, Chi Fu; Chiu, Kou Chou; Cheng, Chao Wen; Huang, Shih Ming; Chen, Pei Hsuan; Chen, Ching Yang; Shieh, Yi Shing.

In: Journal of Dental Sciences, 01.01.2019.

Research output: Contribution to journalArticle

Chen, Szu Yu ; Chiang, Chi Fu ; Chiu, Kou Chou ; Cheng, Chao Wen ; Huang, Shih Ming ; Chen, Pei Hsuan ; Chen, Ching Yang ; Shieh, Yi Shing. / Macrophage phenotypes and Gas6/Axl signaling in apical lesions. In: Journal of Dental Sciences. 2019.
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AU - Chen, Szu Yu

AU - Chiang, Chi Fu

AU - Chiu, Kou Chou

AU - Cheng, Chao Wen

AU - Huang, Shih Ming

AU - Chen, Pei Hsuan

AU - Chen, Ching Yang

AU - Shieh, Yi Shing

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AB - Background/purpose: Macrophages participate in the periapical inflammation with pro-inflammatory M1 cells and anti-inflammatory M2 cells. Gas6/Axl signal is the responsible pathway for the activation of M1 and polarization of M2. The aim of this study was to compare the number of CD16 + M1 cells, CD206 + M2 cells, and Gas6/Axl expression between apical granulomas and radicular cysts. Materials and methods: Twenty-four cases of granuloma and twenty of cysts were submitted to immunohistochemistry using anti-CD16 and anti-CD206 antibodies for determining M1 and M2 macrophages and investigating the cells with positive Gas6 and Axl expression. Results: There were more numerous of M1 macrophages in radicular cysts (175.9 ± 87.7) compared to apical granuloma (116.6 ± 55.8), and M2 macrophages was higher in cysts (204.0 ± 97.6) than granuloma (152.9 ± 64.6). The level of Gas6/Axl expression were similar. There was a significant different in M1 macrophage (P = 0.014) between two diagnosis. In patients with or without root resorption, the number of M1 were 194.6 ± 57.2 compared with 139.1 ± 79.6. The number of M2 were 241.7 ± 81.4 and 164.6 ± 77.1. The expression of Axl was stronger in root resorption patients (191.1 ± 43.6), but the tendency in Gas6 expression was similar. Significant differences were noted in high M2 infiltration and Axl positive lesions. Conclusion: It appears that macrophages associated with significantly higher numbers in radicular cysts than apical granuloma. Meanwhile, macrophages and Axl receptor was intensively expressed in patients with root resorption, related to severe inflammation.

KW - Apical granuloma

KW - Axl

KW - Gas6

KW - Macrophage

KW - Radicular cyst

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