Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma

Yin Hua Shih, Kuo Wei Chang, Michael Yuanchien Chen, Cheng Chia Yu, Dan Jae Lin, Shih Min Hsia, Heng Li Huang, Tzong Ming Shieh

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23 Citations (Scopus)

Abstract

Background Lysyl oxidase (LOX) is a copper-dependent enzyme that cross-links collagen and elastin in the extracellular matrix. LOX overexpressed in various tumors. The manner in which LOX affects tumor growth remains controversial. Methods Chemical treatment and gene transfection were used to induce LOX overexpression or inhibition in cell lines SAS and SVEC4-10. LOX mRNA, protein, and activity were confirmed before tube formation assay and tumorigenesis. The microvessels in the tumor section were detected by immunostaining CD31-positive endothelial cells. Results LOX overexpression and copper induction of LOX activity increased SVEC4-10 tube formation. LOX silencing and β-aminopropionitrile inhibition of LOX activity had opposite effects. LOX overexpression increased proliferation and proliferating cell nuclear antigen expression. High LOX expression clones increased tumor size in a tumorigenesis model. The microvascular numbers were higher in LOX overexpression tumors than in control tumors. Conclusion LOX can induce cell proliferation and angiogenesis in oral squamous cell carcinoma.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalHead and Neck
Volume35
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

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Keywords

  • angiogenesis
  • copper
  • lysyl oxidase
  • microvessel density
  • oral squamous cell carcinoma
  • proliferation

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Shih, Y. H., Chang, K. W., Chen, M. Y., Yu, C. C., Lin, D. J., Hsia, S. M., Huang, H. L., & Shieh, T. M. (2013). Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma. Head and Neck, 35(2), 250-256. https://doi.org/10.1002/hed.22959