Luteolin sensitises drug-resistant human breast cancer cells to tamoxifen via the inhibition of cyclin E2 expression

Shih Hsin Tu, Chi Tang Ho, Ming Fang Liu, Ching Shui Huang, Hui Wen Chang, Chien Hsi Chang, Chih Hsiung Wu, Yuan Soon Ho

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Luteolin is a flavonoid that has been identified in many plant tissues and exhibits chemopreventive or chemosensitising properties against human breast cancer. However, the oncogenic molecules in human breast cancer cells that are inhibited by luteolin treatment have not been identified. This study found that the level of cyclin E2 (CCNE2) mRNA was higher in tumour cells (4.89-fold, *P = 0.005) than in normal paired tissue samples as assessed using real-time reverse-transcriptase polymerase chain reaction (RTPCR) analysis (n = 257). Further, relatively high levels of CCNE2 protein expression were detected in tamoxifen-resistant (TAM-R) MCF-7 cells. These results showed that the level of CCNE2 protein expression was specifically inhibited in luteolin-treated (5 μM) TAM-R cells, either in the presence or absence of 4-OH-TAM (100 nM). Combined treatment with 4-OH-TAM and luteolin synergistically sensitised the TAM-R cells to 4-OH-TAM. The results of this study suggest that luteolin can be used as a chemosensitiser to target the expression level of CCNE2 and that it could be a novel strategy to overcome TAM resistance in breast cancer patients.

Original languageEnglish
Pages (from-to)1553-1561
Number of pages9
JournalFood Chemistry
Volume141
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Luteolin
tamoxifen
Cyclins
luteolin
cyclins
Tamoxifen
breast neoplasms
Cells
Breast Neoplasms
drugs
Pharmaceutical Preparations
protein synthesis
Tissue
Polymerase chain reaction
RNA-Directed DNA Polymerase
MCF-7 Cells
cells
Reverse Transcriptase Polymerase Chain Reaction
Flavonoids
plant tissues

Keywords

  • Breast cancer
  • Cell cycle
  • Cyclin E2
  • Luteolin
  • Tamoxifen

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Luteolin sensitises drug-resistant human breast cancer cells to tamoxifen via the inhibition of cyclin E2 expression. / Tu, Shih Hsin; Ho, Chi Tang; Liu, Ming Fang; Huang, Ching Shui; Chang, Hui Wen; Chang, Chien Hsi; Wu, Chih Hsiung; Ho, Yuan Soon.

In: Food Chemistry, Vol. 141, No. 2, 2013, p. 1553-1561.

Research output: Contribution to journalArticle

Tu, Shih Hsin ; Ho, Chi Tang ; Liu, Ming Fang ; Huang, Ching Shui ; Chang, Hui Wen ; Chang, Chien Hsi ; Wu, Chih Hsiung ; Ho, Yuan Soon. / Luteolin sensitises drug-resistant human breast cancer cells to tamoxifen via the inhibition of cyclin E2 expression. In: Food Chemistry. 2013 ; Vol. 141, No. 2. pp. 1553-1561.
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