Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation

G. E. Hoffman, W. S. Lee, B. Attardi, V. Yann, M. D. Fitzsimmons

Research output: Contribution to journalArticle

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Abstract

Immature female rats received implants containing 17β-estradiol on postnatal day 28 at 0900 h, followed 24 h later by either blank capsules or progesterone. Between 1500-1600 h on the day of progesterone (or blank capsule) implantation, these rats, a group of unoperated or sham controls, and a group of estrogen-progesterone-treated immature male rats were killed and perfused, and their brains processed for immunocytochemistry of c-fos antigen and LHRH. LHRH neurons consistently expressed c-fos after estrogen-progesterone treatment in females but not males; in only one of four females examined was c-fos induced after estrogen treatment. NO fos was associated with LHRH neurons in the control groups. The LHRH neurons that expressed c-fos were located in the preoptic area and anterior hypothalamus; more rostral LHRH cells did not appear stimulated. These data demonstrate that gonadal steroids, administered in a paradigm that predictably produces timed stimulation of LH release, induce c-fos in LHRH neurons. The induction of c-fos in LHRH neurons provides a potentially useful and powerful tool for studying LHRH activation at the cellular level.

Original languageEnglish
Pages (from-to)1736-1741
Number of pages6
JournalEndocrinology
Volume126
Issue number3
Publication statusPublished - Mar 1990
Externally publishedYes

Fingerprint

Gonadotropin-Releasing Hormone
Steroids
Neurons
Antigens
Progesterone
Estrogens
Capsules
Anterior Hypothalamus
Control Groups
Preoptic Area
Estradiol
Immunohistochemistry
Brain
Therapeutics

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hoffman, G. E., Lee, W. S., Attardi, B., Yann, V., & Fitzsimmons, M. D. (1990). Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation. Endocrinology, 126(3), 1736-1741.

Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation. / Hoffman, G. E.; Lee, W. S.; Attardi, B.; Yann, V.; Fitzsimmons, M. D.

In: Endocrinology, Vol. 126, No. 3, 03.1990, p. 1736-1741.

Research output: Contribution to journalArticle

Hoffman, GE, Lee, WS, Attardi, B, Yann, V & Fitzsimmons, MD 1990, 'Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation', Endocrinology, vol. 126, no. 3, pp. 1736-1741.
Hoffman GE, Lee WS, Attardi B, Yann V, Fitzsimmons MD. Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation. Endocrinology. 1990 Mar;126(3):1736-1741.
Hoffman, G. E. ; Lee, W. S. ; Attardi, B. ; Yann, V. ; Fitzsimmons, M. D. / Luteinizing hormone-releasing hormone neurons express c-fos antigen after steroid activation. In: Endocrinology. 1990 ; Vol. 126, No. 3. pp. 1736-1741.
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