5 Citations (Scopus)

Abstract

Src homology-2 domain-containing phosphatase (SHP) 2, an oncogenic phosphatase, inhibits type II immune interferon (IFN)-γ signaling by subverting signal transducers and activators of transcription 1 tyrosine phosphorylation and activation. For cancer immunoediting, this study aimed to investigate the decrease of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor protein, leading to cellular impairment of IFN-γ signaling. In comparison with human lung adenocarcinoma A549 cells, the natural PTEN loss in another human lung adenocarcinoma line, PC14PE6/AS2 cells, presents reduced responsiveness in IFN-γ-induced IFN regulatory factor 1 activation and CD54 expression. Artificially silencing PTEN expression in A549 cells also caused cells to be unresponsive to IFN-γ without affecting IFN-γ receptor expression. IFN-γ-induced inhibition of cell proliferation and cytotoxicity were demonstrated in A549 cells but were defective in PC14PE6/AS2 cells and in PTEN-deficient A549 cells. Aberrant activation of SHP2 by ROS was specifically shown in PC14PE6/AS2 cells and PTEN-deficient A549 cells. Inhibiting ROS and SHP2 rescued cellular responses to IFN-γ-induced cytotoxicity and inhibition of cell proliferation in PC14PE6/AS2 cells. These results demonstrate that a decrease in PTEN facilitates ROS/SHP2 signaling, causing lung cancer cells to become unresponsive to IFN-γ.

Original languageEnglish
Pages (from-to)578-584
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume466
Issue number3
DOIs
Publication statusPublished - Oct 23 2015

Fingerprint

Interferons
Lung Neoplasms
Chemical activation
Cells
Cell proliferation
Cytotoxicity
Phosphoric Monoester Hydrolases
Interferon-gamma
SH2 Domain-Containing Protein Tyrosine Phosphatases
Interferon Regulatory Factor-1
Cell Proliferation
Interferon Receptors
STAT1 Transcription Factor
Tumor Suppressor Proteins
Chromosomes, Human, Pair 10
Phosphorylation
Chromosomes
Tyrosine
A549 Cells
Neoplasms

Keywords

  • IFN-γ
  • PTEN
  • ROS
  • SHP2
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Loss of PTEN causes SHP2 activation, making lung cancer cells unresponsive to IFN-γ. / Chen, Chia-Ling; Chiang, Tzu-Hui; Tseng, Po-Chun; Wang, Yu-Chih; Lin, Chiou Feng.

In: Biochemical and Biophysical Research Communications, Vol. 466, No. 3, 23.10.2015, p. 578-584.

Research output: Contribution to journalArticle

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AU - Chiang, Tzu-Hui

AU - Tseng, Po-Chun

AU - Wang, Yu-Chih

AU - Lin, Chiou Feng

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AB - Src homology-2 domain-containing phosphatase (SHP) 2, an oncogenic phosphatase, inhibits type II immune interferon (IFN)-γ signaling by subverting signal transducers and activators of transcription 1 tyrosine phosphorylation and activation. For cancer immunoediting, this study aimed to investigate the decrease of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor protein, leading to cellular impairment of IFN-γ signaling. In comparison with human lung adenocarcinoma A549 cells, the natural PTEN loss in another human lung adenocarcinoma line, PC14PE6/AS2 cells, presents reduced responsiveness in IFN-γ-induced IFN regulatory factor 1 activation and CD54 expression. Artificially silencing PTEN expression in A549 cells also caused cells to be unresponsive to IFN-γ without affecting IFN-γ receptor expression. IFN-γ-induced inhibition of cell proliferation and cytotoxicity were demonstrated in A549 cells but were defective in PC14PE6/AS2 cells and in PTEN-deficient A549 cells. Aberrant activation of SHP2 by ROS was specifically shown in PC14PE6/AS2 cells and PTEN-deficient A549 cells. Inhibiting ROS and SHP2 rescued cellular responses to IFN-γ-induced cytotoxicity and inhibition of cell proliferation in PC14PE6/AS2 cells. These results demonstrate that a decrease in PTEN facilitates ROS/SHP2 signaling, causing lung cancer cells to become unresponsive to IFN-γ.

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