Long-lasting auditory gating deficit accompanied by GABA B receptor dysfunction in the hippocampus after early-life limbic seizures in rats

Min Lan Tsai, Melanie Crutchley, Richard Boyce, Jingyi Ma, Francis Boon, D. Peter Cain, L. Stan Leung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In a previous study, we reported a rat model of early-life limbic seizures which resulted in a loss of GABA B receptor inhibition in the hippocampus. Since gating of auditory evoked potentials in the hippocampus (auditory gating) requires GABA B receptors and spatial behaviors depend on the hippocampus, we hypothesize that rats with early-life limbic seizures manifest deficits of auditory gating and spatial behaviors. Seizure rats were given a single injection of GABA B receptor antagonist CGP56999A (1-1.2mg/kg i.p.) on postnatal day (PND) 15, which induced multiple limbic seizures in 8h; control rats were given saline injection. When tested at 3-9weeks after seizure/control treatment, seizure as compared to control rats showed no difference in finding a hidden platform in the water maze, but were deficient in learning and maintaining consecutive criterion performance in the 8-arm radial arm maze. Auditory gating, as measured by paired-click (conditioning followed by test click) average auditory evoked potentials in the hippocampus, revealed a significant difference between seizure rats and controls. Seizure as compared to control rats showed an increased ratio of the test to conditioning click response as adolescents (50days old) or adults (70days old). Heterosynaptic electric paired-pulse depression of hippocampal population excitatory postsynaptic potential in freely moving rats, a measure of hippocampal GABA B-receptor mediated inhibition, was decreased in seizure as compared to control rats. Seizure as compared to control rats showed increased locomotor activity in a novel open field for the first 10min, and decreased activity at 15-60min. However, auditory prepulse inhibition, a measure of sensorimotor gating, revealed no difference between seizure and control rats. In conclusion, early-life limbic seizures induced a long-lasting deficit in auditory gating, likely caused by GABA B receptor-mediated inhibition loss in the hippocampus. Auditory gating loss is a symptom of schizophrenia, and thus GABA B receptor inhibition loss in the hippocampus provides a mechanism linking early-life seizures to a psychiatric symptom.

Original languageEnglish
Pages (from-to)534-541
Number of pages8
JournalPhysiology and Behavior
Volume106
Issue number4
DOIs
Publication statusPublished - Jun 25 2012
Externally publishedYes

Fingerprint

GABA-B Receptors
Hippocampus
Seizures
Spatial Behavior
Auditory Evoked Potentials
GABA-B Receptor Antagonists
Sensory Gating
Injections
Excitatory Postsynaptic Potentials
Locomotion

Keywords

  • Auditory gating
  • Early-life seizures
  • GABA receptor inhibition
  • Prepulse inhibition
  • Radial arm maze
  • Water maze

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Long-lasting auditory gating deficit accompanied by GABA B receptor dysfunction in the hippocampus after early-life limbic seizures in rats. / Tsai, Min Lan; Crutchley, Melanie; Boyce, Richard; Ma, Jingyi; Boon, Francis; Cain, D. Peter; Leung, L. Stan.

In: Physiology and Behavior, Vol. 106, No. 4, 25.06.2012, p. 534-541.

Research output: Contribution to journalArticle

Tsai, Min Lan ; Crutchley, Melanie ; Boyce, Richard ; Ma, Jingyi ; Boon, Francis ; Cain, D. Peter ; Leung, L. Stan. / Long-lasting auditory gating deficit accompanied by GABA B receptor dysfunction in the hippocampus after early-life limbic seizures in rats. In: Physiology and Behavior. 2012 ; Vol. 106, No. 4. pp. 534-541.
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