Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep

C. D'Anaelis, C. Tzao, F. C. Morin, L. M. Wild, R. H. Steinhorn, P. A. Nickerson

Research output: Contribution to journalArticle

Abstract

Activation of sGC by Nitric Oxide (NO) is essential for the pulmonary vasodilation that allows the establishment of gas exchange by the lungs at birth. The relative role of each segment of the pulmonary vasculature of the newborn in this activation of sGC is not known. Immunohistochemistry using the 64 antibody to the 82 kDA subunit of rat sGC (cross reacts with the sheep) was used to study differences along the pulmonary vasculature in fetal sheep. Specific staining was confined to vascular smooth muscle layers. At 1:6400 dilution, al! veins stained positively, whereas the largest positively stained artery associated with the terminal bronchiole was 200|.im. All vessels at the level of small terminal and respiratory bronchioles and alveolar ducts stained intensely positive. This pattern of differential staining between small and large pulmonary arteries supports previous physiologic studies in fetal sheep lung showing decreased sensitivity to NO in pulmonary arteries greater than 500 μm in diameter but sustained activity in pulmonary veins. Our data supports the role for sGC activation in the intraacinar arteries and veins of the newbron pulmonary circulation.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

guanylate cyclase
Guanylate Cyclase
Sheep
Bronchioles
Chemical activation
lungs
sheep
Lung
Nitric Oxide
Pulmonary Artery
Veins
pulmonary artery
Ducts
Arteries
Dilution
Muscle
Rats
Staining and Labeling
arteries
nitric oxide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

D'Anaelis, C., Tzao, C., Morin, F. C., Wild, L. M., Steinhorn, R. H., & Nickerson, P. A. (1996). Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep. FASEB Journal, 10(3).

Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep. / D'Anaelis, C.; Tzao, C.; Morin, F. C.; Wild, L. M.; Steinhorn, R. H.; Nickerson, P. A.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

D'Anaelis, C, Tzao, C, Morin, FC, Wild, LM, Steinhorn, RH & Nickerson, PA 1996, 'Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep', FASEB Journal, vol. 10, no. 3.
D'Anaelis C, Tzao C, Morin FC, Wild LM, Steinhorn RH, Nickerson PA. Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep. FASEB Journal. 1996;10(3).
D'Anaelis, C. ; Tzao, C. ; Morin, F. C. ; Wild, L. M. ; Steinhorn, R. H. ; Nickerson, P. A. / Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
@article{4435fc9fc2314e6393b213964be788c5,
title = "Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep",
abstract = "Activation of sGC by Nitric Oxide (NO) is essential for the pulmonary vasodilation that allows the establishment of gas exchange by the lungs at birth. The relative role of each segment of the pulmonary vasculature of the newborn in this activation of sGC is not known. Immunohistochemistry using the 64 antibody to the 82 kDA subunit of rat sGC (cross reacts with the sheep) was used to study differences along the pulmonary vasculature in fetal sheep. Specific staining was confined to vascular smooth muscle layers. At 1:6400 dilution, al! veins stained positively, whereas the largest positively stained artery associated with the terminal bronchiole was 200|.im. All vessels at the level of small terminal and respiratory bronchioles and alveolar ducts stained intensely positive. This pattern of differential staining between small and large pulmonary arteries supports previous physiologic studies in fetal sheep lung showing decreased sensitivity to NO in pulmonary arteries greater than 500 μm in diameter but sustained activity in pulmonary veins. Our data supports the role for sGC activation in the intraacinar arteries and veins of the newbron pulmonary circulation.",
author = "C. D'Anaelis and C. Tzao and Morin, {F. C.} and Wild, {L. M.} and Steinhorn, {R. H.} and Nickerson, {P. A.}",
year = "1996",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Localization of soluble guanylate cyclase (SGC) in pulmonary vasculature of fetal sheep

AU - D'Anaelis, C.

AU - Tzao, C.

AU - Morin, F. C.

AU - Wild, L. M.

AU - Steinhorn, R. H.

AU - Nickerson, P. A.

PY - 1996

Y1 - 1996

N2 - Activation of sGC by Nitric Oxide (NO) is essential for the pulmonary vasodilation that allows the establishment of gas exchange by the lungs at birth. The relative role of each segment of the pulmonary vasculature of the newborn in this activation of sGC is not known. Immunohistochemistry using the 64 antibody to the 82 kDA subunit of rat sGC (cross reacts with the sheep) was used to study differences along the pulmonary vasculature in fetal sheep. Specific staining was confined to vascular smooth muscle layers. At 1:6400 dilution, al! veins stained positively, whereas the largest positively stained artery associated with the terminal bronchiole was 200|.im. All vessels at the level of small terminal and respiratory bronchioles and alveolar ducts stained intensely positive. This pattern of differential staining between small and large pulmonary arteries supports previous physiologic studies in fetal sheep lung showing decreased sensitivity to NO in pulmonary arteries greater than 500 μm in diameter but sustained activity in pulmonary veins. Our data supports the role for sGC activation in the intraacinar arteries and veins of the newbron pulmonary circulation.

AB - Activation of sGC by Nitric Oxide (NO) is essential for the pulmonary vasodilation that allows the establishment of gas exchange by the lungs at birth. The relative role of each segment of the pulmonary vasculature of the newborn in this activation of sGC is not known. Immunohistochemistry using the 64 antibody to the 82 kDA subunit of rat sGC (cross reacts with the sheep) was used to study differences along the pulmonary vasculature in fetal sheep. Specific staining was confined to vascular smooth muscle layers. At 1:6400 dilution, al! veins stained positively, whereas the largest positively stained artery associated with the terminal bronchiole was 200|.im. All vessels at the level of small terminal and respiratory bronchioles and alveolar ducts stained intensely positive. This pattern of differential staining between small and large pulmonary arteries supports previous physiologic studies in fetal sheep lung showing decreased sensitivity to NO in pulmonary arteries greater than 500 μm in diameter but sustained activity in pulmonary veins. Our data supports the role for sGC activation in the intraacinar arteries and veins of the newbron pulmonary circulation.

UR - http://www.scopus.com/inward/record.url?scp=33748963439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748963439&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -