Localization of D-aspartic acid in elongate spermatids in rat testis

Kumiko Sakai, Hiroshi Homma, Jen Ai Lee, Takeshi Fukushima, Tomofumi Santa, Ken Tashiro, Takeshi Iwatsubo, Kazuhiro Imai

Research output: Contribution to journalArticle

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Abstract

In the current study, localization of D-aspartic acid (D-Asp) in rat testis was studied by immunohistochemical and biochemical techniques. Immunohistochemical staining of this tissue using specific polyclonal antibody to D-Asp revealed D-Asp immunoreactivity (IR) in the cytoplasm of germ cells, especially around the region rich in elongate spermatids, the most mature of the germ cells. Weak IR was also noted in cytoplasm of spermatocytes and round spermatids; however, it was negligible in interstitial cells and Sertoli cells. The intensity of immunostaining in each seminiferous tubule differed according to its distinct germ cell composition. In testis of young rats, seminiferous tubules lack elongated spermatids, and D-Asp was found to be localized in spermatocytes, the most mature population of germ cells at that age. We used various toxicants to destroy specific testicular cell populations and to confirm the localization of D-Asp in rat testis. Administration of ethane dimethane sulfonate induced a selective destruction of all Leydig cells in this tissue. This resulted in a significant decrease in the D-Asp level, which was probably due to a drop in testosterone brought about by this treatment, and this was followed by a modulation of spermatogenesis. Three days after treatment with methoxyacetic acid (MAA), many seminiferous tubules were found to lack or to have severe depletions of pachytene spermatocytes, but not of elongate spermatids. This caused reductions in protein content and in the total amount of L-Asp, but not that of D-Asp. Twenty days after treatment with MAA, the depleted population of germ cells progressed through the spermatogenic cycle from pachytene spermatocytes to elongate spermatids. At this time, the level of D- Asp decreased significantly, as did that of L-Asp and protein, consistent with D-Asp localization in elongate spermatids. This decrease in the D-Asp level was also seen with immunostaining.

Original languageEnglish
Pages (from-to)96-105
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume351
Issue number1
DOIs
Publication statusPublished - Mar 1 1998
Externally publishedYes

Fingerprint

D-Aspartic Acid
Spermatids
Testis
Rats
Germ Cells
Spermatocytes
Cells
Seminiferous Tubules
Cytoplasm
Tissue
Population
Leydig Cells
Sertoli Cells
Spermatogenesis
Testosterone
Proteins
Modulation
Staining and Labeling
Antibodies
Chemical analysis

Keywords

  • D-aspartate
  • D-aspartic acid
  • D-mino acids
  • EDS (ethane dimethane sulfonate)
  • Epididymis
  • Immunohistochemistry
  • MAA (methoxyacetic acid)
  • Rat
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization of D-aspartic acid in elongate spermatids in rat testis. / Sakai, Kumiko; Homma, Hiroshi; Lee, Jen Ai; Fukushima, Takeshi; Santa, Tomofumi; Tashiro, Ken; Iwatsubo, Takeshi; Imai, Kazuhiro.

In: Archives of Biochemistry and Biophysics, Vol. 351, No. 1, 01.03.1998, p. 96-105.

Research output: Contribution to journalArticle

Sakai, K, Homma, H, Lee, JA, Fukushima, T, Santa, T, Tashiro, K, Iwatsubo, T & Imai, K 1998, 'Localization of D-aspartic acid in elongate spermatids in rat testis', Archives of Biochemistry and Biophysics, vol. 351, no. 1, pp. 96-105. https://doi.org/10.1006/abbi.1997.0539
Sakai, Kumiko ; Homma, Hiroshi ; Lee, Jen Ai ; Fukushima, Takeshi ; Santa, Tomofumi ; Tashiro, Ken ; Iwatsubo, Takeshi ; Imai, Kazuhiro. / Localization of D-aspartic acid in elongate spermatids in rat testis. In: Archives of Biochemistry and Biophysics. 1998 ; Vol. 351, No. 1. pp. 96-105.
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