Localization and quantification of endoplasmic reticulum Ca2+-ATPase isoform transcripts

K. D. Wu, W. S. Lee, J. Wey, D. Bungard, J. Lytton

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The Ca2+-adenosinetriphosphatase pump of the sarcoplasmic or endoplasmic reticulum (SERCA) plays a critical role in Ca2+ signaling and homeostasis in all cells and is encoded by a family of homologous and alternatively spliced genes. To understand more clearly the role the different isoforms play in cell physiology, we have undertaken a quantitative and qualitative assessment of the tissue distribution of transcripts encoding each SERCA isoform. SERCA1 expression is restricted to fast-twitch striated muscles, SERCA2a to cardiac and slow-twitch striated muscles, whereas SERCA2b is ubiquitously expressed. SERCA3 is expressed most abundantly in large and small intestine, thymus, and cerebellum and at lower levels in spleen, lymph node, and lung. In situ hybridization analyses revealed SERCA3 transcripts in cells of the intestinal crypt, the thymic cortex, and Purkinje cells in cerebellum. In addition, SERCA3 was expressed abundantly in isolated rat spleen lymphocytes, in various murine lymphoid cell lines, and in primary cultured microvascular endothelial cells. This analysis demonstrates that SERCA3 is expressed selectively in cells in which Ca2+ signaling plays a critical and sensitive role in regulating physiological processes.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number3 38-3
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Ca2-transporting ATPase
Calcium-Transporting ATPases
Endoplasmic Reticulum
endoplasmic reticulum
Muscle
Protein Isoforms
Striated Muscle
Thymus
Cerebellum
striated muscle
Recombinant DNA
Lymphocytes
Endothelial cells
Physiology
cerebellum
Spleen
calcium
Physiological Phenomena
Adenosine Triphosphatases
Rats

Keywords

  • in situ hybridization
  • quantitative Northern blotting
  • sarcoplasmic or endoplasmic reticulum calcium-adenosinetriphosphatase isoforms

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Localization and quantification of endoplasmic reticulum Ca2+-ATPase isoform transcripts. / Wu, K. D.; Lee, W. S.; Wey, J.; Bungard, D.; Lytton, J.

In: American Journal of Physiology - Cell Physiology, Vol. 269, No. 3 38-3, 1995.

Research output: Contribution to journalArticle

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