Local pH tracking in living cells

Chieh Jui Tsou, Chih Hao Hsia, Jia Yin Chu, Yann Hung, Yi Ping Chen, Fan Ching Chien, Keng C. Chou, Peilin Chen, Chung Yuan Mou

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells were demonstrated for the first time by combining fluorescent mesoporous silica nanoparticles (FMSNs) and a single-particle tracking (SPT) technique with a precision of ∼10 nm. FMSNs, synthesized by the co-condensation of both pH-sensitive and reference dyes with a silica/surfactant source, allow long-term reliable ratiometric pH measurements with a precision better than 0.3 pH unit because of their excellent brightness and stability. pH variation in the surrounding area of FMSNs during endocytosis was monitored in real-time. Acidification and low mobility of FMSNs were observed at the early endocytic stage, whereas basification and high mobility of FMSNs were observed at the late stage. Our results indicate that it is possible to monitor local pH changes in the environments surrounding nanoparticles during the cellular uptake process of FMSNs, which provides much needed information for designing an efficient drug delivery nanosystem. This journal is

Original languageEnglish
Pages (from-to)4217-4225
Number of pages9
JournalNanoscale
Volume7
Issue number9
DOIs
Publication statusPublished - Mar 7 2015
Externally publishedYes

Fingerprint

Silicon Dioxide
Silica
Cells
Nanoparticles
Nanosystems
Acidification
Drug delivery
Surface-Active Agents
Luminance
Condensation
Surface active agents
Coloring Agents
Dyes

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Tsou, C. J., Hsia, C. H., Chu, J. Y., Hung, Y., Chen, Y. P., Chien, F. C., ... Mou, C. Y. (2015). Local pH tracking in living cells. Nanoscale, 7(9), 4217-4225. https://doi.org/10.1039/c4nr06545j

Local pH tracking in living cells. / Tsou, Chieh Jui; Hsia, Chih Hao; Chu, Jia Yin; Hung, Yann; Chen, Yi Ping; Chien, Fan Ching; Chou, Keng C.; Chen, Peilin; Mou, Chung Yuan.

In: Nanoscale, Vol. 7, No. 9, 07.03.2015, p. 4217-4225.

Research output: Contribution to journalArticle

Tsou, CJ, Hsia, CH, Chu, JY, Hung, Y, Chen, YP, Chien, FC, Chou, KC, Chen, P & Mou, CY 2015, 'Local pH tracking in living cells', Nanoscale, vol. 7, no. 9, pp. 4217-4225. https://doi.org/10.1039/c4nr06545j
Tsou CJ, Hsia CH, Chu JY, Hung Y, Chen YP, Chien FC et al. Local pH tracking in living cells. Nanoscale. 2015 Mar 7;7(9):4217-4225. https://doi.org/10.1039/c4nr06545j
Tsou, Chieh Jui ; Hsia, Chih Hao ; Chu, Jia Yin ; Hung, Yann ; Chen, Yi Ping ; Chien, Fan Ching ; Chou, Keng C. ; Chen, Peilin ; Mou, Chung Yuan. / Local pH tracking in living cells. In: Nanoscale. 2015 ; Vol. 7, No. 9. pp. 4217-4225.
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