Local anesthetics induce apoptosis in human breast tumor cells

Yuan Ching Chang, Chien Liang Liu, Ming Jen Chen, Yung Wei Hsu, Shan Na Chen, Chi Hsin Lin, Chin Man Chen, Feng Ming Yang, Meng Chun Hu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

BACKGROUND:: Previous studies have shown that local anesthetics may induce apoptosis in some cell types. In this study, we investigated the apoptotic effects of local anesthetics in human breast tumor cells. METHODS:: Human breast cancer (MCF-7) and mammary epithelial (MCF-10A) cell lines were treated with lidocaine and/or bupivacaine. Cell viability, DNA fragmentation, and annexin V immunofluorescence staining were assessed. The effects on apoptosis-related protein expression were investigated by Western blot analysis. The findings were extended to studies in an in vivo xenograft model. RESULTS:: Treatment of breast tumor cells with lidocaine and bupivacaine resulted in inhibition of cell viability via induction of apoptosis. The effects were more prominent in MCF-7 cells than in MCF-10A cells. Treatment with local anesthetics induced caspase 7, 8, 9, and poly ADP-ribose polymerase cleavage. The cleavage of caspase 7 and poly ADP-ribose polymerase induced by local anesthetics were effectively blocked by caspase inhibitors. Furthermore, treatment of MCF-7 xenografts with local anesthetics resulted in higher expression of cleaved caspase 7 and an increase in terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. CONCLUSION:: Lidocaine and bupivacaine induce apoptosis of breast tumor cells at clinically relevant concentrations. Our results reveal previously unrecognized beneficial actions of local anesthetics and call for further studies to assess the oncologic advantages of their use during breast cancer surgery.

Original languageEnglish
Pages (from-to)116-124
Number of pages9
JournalAnesthesia and Analgesia
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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Local Anesthetics
Apoptosis
Breast Neoplasms
Caspase 7
Bupivacaine
Lidocaine
Poly(ADP-ribose) Polymerases
Heterografts
Cell Survival
Staining and Labeling
Caspase Inhibitors
DNA Nucleotidylexotransferase
Caspase 8
Annexin A5
MCF-7 Cells
DNA Fragmentation
Fluorescent Antibody Technique
Breast
Therapeutics
Western Blotting

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Chang, Y. C., Liu, C. L., Chen, M. J., Hsu, Y. W., Chen, S. N., Lin, C. H., ... Hu, M. C. (2014). Local anesthetics induce apoptosis in human breast tumor cells. Anesthesia and Analgesia, 118(1), 116-124. https://doi.org/10.1213/ANE.0b013e3182a94479

Local anesthetics induce apoptosis in human breast tumor cells. / Chang, Yuan Ching; Liu, Chien Liang; Chen, Ming Jen; Hsu, Yung Wei; Chen, Shan Na; Lin, Chi Hsin; Chen, Chin Man; Yang, Feng Ming; Hu, Meng Chun.

In: Anesthesia and Analgesia, Vol. 118, No. 1, 01.01.2014, p. 116-124.

Research output: Contribution to journalArticle

Chang, YC, Liu, CL, Chen, MJ, Hsu, YW, Chen, SN, Lin, CH, Chen, CM, Yang, FM & Hu, MC 2014, 'Local anesthetics induce apoptosis in human breast tumor cells', Anesthesia and Analgesia, vol. 118, no. 1, pp. 116-124. https://doi.org/10.1213/ANE.0b013e3182a94479
Chang YC, Liu CL, Chen MJ, Hsu YW, Chen SN, Lin CH et al. Local anesthetics induce apoptosis in human breast tumor cells. Anesthesia and Analgesia. 2014 Jan 1;118(1):116-124. https://doi.org/10.1213/ANE.0b013e3182a94479
Chang, Yuan Ching ; Liu, Chien Liang ; Chen, Ming Jen ; Hsu, Yung Wei ; Chen, Shan Na ; Lin, Chi Hsin ; Chen, Chin Man ; Yang, Feng Ming ; Hu, Meng Chun. / Local anesthetics induce apoptosis in human breast tumor cells. In: Anesthesia and Analgesia. 2014 ; Vol. 118, No. 1. pp. 116-124.
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