LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip

Chih-Shen Chuang, Chieh-Ying Wu, Po-Han Juan, Nai-Cheng Hou, Yu-Jui Fan, Pei-Kuen Wei, Horn-Jiunn Sheen

Research output: Contribution to journalArticle

Abstract

Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU−1 and the detection limit was 4.1 × 10−5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip.
Original languageTraditional Chinese
JournalAnalyst
DOIs
Publication statusPublished - 2020

Cite this

LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip. / Chuang, Chih-Shen; Wu, Chieh-Ying; Juan, Po-Han; Hou, Nai-Cheng; Fan, Yu-Jui; Wei, Pei-Kuen; Sheen, Horn-Jiunn.

In: Analyst, 2020.

Research output: Contribution to journalArticle

Chuang, Chih-Shen ; Wu, Chieh-Ying ; Juan, Po-Han ; Hou, Nai-Cheng ; Fan, Yu-Jui ; Wei, Pei-Kuen ; Sheen, Horn-Jiunn. / LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip. In: Analyst. 2020.
@article{aae592418a71493b9cc5e36c06b7786f,
title = "LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip",
abstract = "Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU−1 and the detection limit was 4.1 × 10−5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip.",
author = "Chih-Shen Chuang and Chieh-Ying Wu and Po-Han Juan and Nai-Cheng Hou and Yu-Jui Fan and Pei-Kuen Wei and Horn-Jiunn Sheen",
year = "2020",
doi = "10.1039/C9AN01419E",
language = "繁體中文",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip

AU - Chuang, Chih-Shen

AU - Wu, Chieh-Ying

AU - Juan, Po-Han

AU - Hou, Nai-Cheng

AU - Fan, Yu-Jui

AU - Wei, Pei-Kuen

AU - Sheen, Horn-Jiunn

PY - 2020

Y1 - 2020

N2 - Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU−1 and the detection limit was 4.1 × 10−5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip.

AB - Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU−1 and the detection limit was 4.1 × 10−5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip.

U2 - 10.1039/C9AN01419E

DO - 10.1039/C9AN01419E

M3 - 文章

JO - The Analyst

JF - The Analyst

SN - 0003-2654

ER -