Liver cancer cells

Targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres

Yu Te Liao, Chia Hung Liu, Jiashing Yu, Kevin C.W. Wu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate (denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the (lysine)4-tyrosine-arginine-glycine-aspartic acid (K4YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.

Original languageEnglish
Pages (from-to)2767-2778
Number of pages12
JournalInternational Journal of Nanomedicine
Volume9
Issue number1
DOIs
Publication statusPublished - Jun 5 2014

Fingerprint

Drug Carriers
Alginate
Liver Neoplasms
Microspheres
Silicon Dioxide
Liver
Nanoparticles
Silica
Cells
Drug Delivery Systems
Arginine
Biocompatibility
Aspartic Acid
Pharmaceutical Preparations
Doxorubicin
Glycine
Amino acids
alginic acid
Acids
Atomization

Keywords

  • Alginate
  • Atomization
  • Mesoporous silica nanoparticles
  • Sustained release
  • Targeting therapy

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Liver cancer cells : Targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres. / Liao, Yu Te; Liu, Chia Hung; Yu, Jiashing; Wu, Kevin C.W.

In: International Journal of Nanomedicine, Vol. 9, No. 1, 05.06.2014, p. 2767-2778.

Research output: Contribution to journalArticle

@article{4e5712c215254f06aff5e768b25c69e3,
title = "Liver cancer cells: Targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres",
abstract = "A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate (denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the (lysine)4-tyrosine-arginine-glycine-aspartic acid (K4YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.",
keywords = "Alginate, Atomization, Mesoporous silica nanoparticles, Sustained release, Targeting therapy",
author = "Liao, {Yu Te} and Liu, {Chia Hung} and Jiashing Yu and Wu, {Kevin C.W.}",
year = "2014",
month = "6",
day = "5",
doi = "10.2147/IJN.S60171",
language = "English",
volume = "9",
pages = "2767--2778",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Liver cancer cells

T2 - Targeting and prolonged-release drug carriers consisting of mesoporous silica nanoparticles and alginate microspheres

AU - Liao, Yu Te

AU - Liu, Chia Hung

AU - Yu, Jiashing

AU - Wu, Kevin C.W.

PY - 2014/6/5

Y1 - 2014/6/5

N2 - A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate (denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the (lysine)4-tyrosine-arginine-glycine-aspartic acid (K4YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.

AB - A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate (denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the (lysine)4-tyrosine-arginine-glycine-aspartic acid (K4YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.

KW - Alginate

KW - Atomization

KW - Mesoporous silica nanoparticles

KW - Sustained release

KW - Targeting therapy

UR - http://www.scopus.com/inward/record.url?scp=84901990086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901990086&partnerID=8YFLogxK

U2 - 10.2147/IJN.S60171

DO - 10.2147/IJN.S60171

M3 - Article

VL - 9

SP - 2767

EP - 2778

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

IS - 1

ER -