Live and heat-killed Lactobacillus rhamnosus GG upregulate gene expression of pro-inflammatory cytokines in 5-fluorouracil-pretreated Caco-2 cells

Shiuh Bin Fang, Hsin Yu Shih, Chih Hung Huang, Li Ting Li, Chia Chun Chen, Hsu Wei Fang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: This study investigates whether post-chemotherapeutic use of live and heat-killed Lactobacillus rhamnosus GG can modulate the expression of three pro-inflammatory cytokines in 5-fluorouracil (5-FU)-induced intestinal mucositis in vitro. Methods: Live L. rhamnosus GG and heat-killed L. rhamnosus GG were observed using scanning electron microscopy. To establish the duration required for optimal expression of tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1), and interleukin-12 (IL-12), 5 μM of 5-FU was selected to treat 10-day-old Caco-2 cells for 4, 6, 8, and 24 h. Caco-2 cells were treated with 5-FU (5 μM) for 4 h, followed by the administration of live L. rhamnosus GG (multiplicity of infection=25), and heat-killed L. rhamnosus GG for 2 and 4 h. Finally, total cellular RNA was isolated to quantify mRNA expression of TNF-α, MCP-1, and IL-12 using real-time PCR. Results: The results demonstrated that heat-killed L. rhamnosus GG remained structurally intact with elongation. A biphasic upregulated expression of TNF-α, MCP-1, and IL-12 was observed in 5-FU-treated Caco-2 cells at 4 and 24 h. Compared to non-L. rhamnosus GG controls in 5-FU-pretreated Caco-2 cells, a 2-h treatment of heat-killed L. rhamnosus GG significantly upregulated the MCP-1 expression (p

Original languageEnglish
Pages (from-to)1647-1654
Number of pages8
JournalSupportive Care in Cancer
Volume22
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Lactobacillus rhamnosus
Caco-2 Cells
Fluorouracil
Up-Regulation
Hot Temperature
Cytokines
Gene Expression
Chemokine CCL2
Interleukin-12
Tumor Necrosis Factor-alpha
Mucositis
Interleukin-5

Keywords

  • 5-Fluorouracil
  • Human intestinal epithelium
  • Interleukin-12
  • Lactobacillus rhamnosus GG
  • Monocyte chemotactic protein-1
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Oncology

Cite this

Live and heat-killed Lactobacillus rhamnosus GG upregulate gene expression of pro-inflammatory cytokines in 5-fluorouracil-pretreated Caco-2 cells. / Fang, Shiuh Bin; Shih, Hsin Yu; Huang, Chih Hung; Li, Li Ting; Chen, Chia Chun; Fang, Hsu Wei.

In: Supportive Care in Cancer, Vol. 22, No. 6, 2014, p. 1647-1654.

Research output: Contribution to journalArticle

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abstract = "Purpose: This study investigates whether post-chemotherapeutic use of live and heat-killed Lactobacillus rhamnosus GG can modulate the expression of three pro-inflammatory cytokines in 5-fluorouracil (5-FU)-induced intestinal mucositis in vitro. Methods: Live L. rhamnosus GG and heat-killed L. rhamnosus GG were observed using scanning electron microscopy. To establish the duration required for optimal expression of tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1), and interleukin-12 (IL-12), 5 μM of 5-FU was selected to treat 10-day-old Caco-2 cells for 4, 6, 8, and 24 h. Caco-2 cells were treated with 5-FU (5 μM) for 4 h, followed by the administration of live L. rhamnosus GG (multiplicity of infection=25), and heat-killed L. rhamnosus GG for 2 and 4 h. Finally, total cellular RNA was isolated to quantify mRNA expression of TNF-α, MCP-1, and IL-12 using real-time PCR. Results: The results demonstrated that heat-killed L. rhamnosus GG remained structurally intact with elongation. A biphasic upregulated expression of TNF-α, MCP-1, and IL-12 was observed in 5-FU-treated Caco-2 cells at 4 and 24 h. Compared to non-L. rhamnosus GG controls in 5-FU-pretreated Caco-2 cells, a 2-h treatment of heat-killed L. rhamnosus GG significantly upregulated the MCP-1 expression (p",
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AU - Fang, Shiuh Bin

AU - Shih, Hsin Yu

AU - Huang, Chih Hung

AU - Li, Li Ting

AU - Chen, Chia Chun

AU - Fang, Hsu Wei

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