Liquid chromatographic assay of phenothiazine, thioxanthene and butyrophenone neuroleptics and antihistamines in blood and plasma with conventional and radial compression columns and UV and electrochemical detection

Stephen H. Curry, Edgar A. Brown, Oliver Y.P. Hu, John H. Perrin

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

An assay strategy for determining a wide range of phenothiazine, thioxanthene and butyrophenone neuroleptics and antihistamines both alone and in combination in blood and plasma is described. The general method employs liquid chromatography with both conventional and radial compression nitrile bonded columns. Detection is by ultraviolet absorption spectrophotometry or by amperometry depending on the concentrations to be measured. Ultraviolet absorption is suitable down to 10 ng/ml. Below this level amperometry is preferable. The various compounds are used as internal standards for each other. The lower limit of detection is approximately 0.1 ng ml-1 with a 10-ml sample. The within-run coefficient of variation is a maximum of 7.3%.

Original languageEnglish
Pages (from-to)361-376
Number of pages16
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume231
Issue number2
DOIs
Publication statusPublished - Sep 10 1982
Externally publishedYes

Fingerprint

Thioxanthenes
Butyrophenones
Nitriles
Histamine Antagonists
Liquid chromatography
Spectrophotometry
Antipsychotic Agents
Assays
Blood
Plasmas
Liquids
phenothiazine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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