An assay strategy for determining a wide range of phenothiazine, thioxanthene and butyrophenone neuroleptics and antihistamines both alone and in combination in blood and plasma is described. The general method employs liquid chromatography with both conventional and radial compression nitrile bonded columns. Detection is by ultraviolet absorption spectrophotometry or by amperometry depending on the concentrations to be measured. Ultraviolet absorption is suitable down to 10 ng/ml. Below this level amperometry is preferable. The various compounds are used as internal standards for each other. The lower limit of detection is approximately 0.1 ng ml-1 with a 10-ml sample. The within-run coefficient of variation is a maximum of 7.3%.
|Number of pages||16|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - Sep 10 1982|
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