Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer

Anthony I. Magee, Antonios D. Konitsiotis, Biljana Jovanovic, Shu-Chun Chang, Paulina Ciepla, Naoko Masumoto, Edward W. Tate

Research output: Contribution to journalReview article

Abstract

Hedgehog (Hh) proteins (Shh, Dhh and Ihh) are secreted intercellular signalling molecules important in mammalian development and also in the aetiology of many human cancers, hence the Hh signalling pathway is a popular target for development of anti-cancer therapies (1). Hhs are unique in being dually lipidated with a N-terminal amide-linked palmitate and a C-terminal cholesterol, added during its transit through the secretory pathway. These modifications are crucial for assembly into macromolecular complexes for cell-to-cell transport and also for biological function; non-lipidated Hhs are dramatically less potent than the fully lipidated protein.

Hedgehog acyltransferase (Hhat) is the enzyme responsible for palmitoylation of the N-terminal cysteine of Hh proteins, and belongs to the membrane bound O-acyltransferase (MBOAT) protein family. Knockdown experiments in pancreatic ductal adenocarcinoma and non-small cell lung cancer cells demonstrate that Hhat is crucial for Shh palmitoylation, assembly into macromolecular complexes and function as assessed by Hh pathway activation, cell growth and invasion, and para/juxtacrine signalling (2). Our results underline the potential of this enzyme as a target for chemotherapy in diverse human cancers including PDAC.

Our work has been greatly facilitated by development of bioorthogonal chemical labels for the palmitate and cholesterol moieties (3). Recent results using these to study novel inhibitors will be presented. Hhat is predicted to have 8-12 transmembrane domains but the true number is not known. We have made recent progress in analysis of the membrane topology of Hhat which will allow us better to understand how it functions, and aid in inhibitor screening.

1. Dlugosz AA, Talpaz M. Following the hedgehog to new cancer therapies. N Engl J Med 2009; 361: 1202-5.

2. Konitsiotis AK, Chang SC, Jovanovic B, Masumoto N, Ciepla P, Palmer CP et al. Attenuation of Hedgehog acyltransferase-catalyzed Sonic hedgehog palmitoylation causes reduced proliferation and invasiveness of human carcinoma cells. PLoSONE in press.

3. Heal WP, Jovanovic B, Bessin S, Wright MH, Magee AI and Tate EW. Bioorthogonal chemical tagging of protein cholesterylation in living cells. Chem Comm 2011; 47: 4081-3.
Original languageEnglish
Pages (from-to)e4
JournalPancreatology
Volume14
Issue number2
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Hedgehog Proteins
Acyltransferases
Pancreatic Neoplasms
Lipoylation
Macromolecular Substances
Palmitates
Neoplasms
Therapeutics
Biological Transport
Cholesterol
Membranes
Secretory Pathway
Enzymes
Amides
Non-Small Cell Lung Carcinoma
Cysteine
Proteins
Adenocarcinoma
Carcinoma
Drug Therapy

Cite this

Magee, A. I., Konitsiotis, A. D., Jovanovic, B., Chang, S-C., Ciepla, P., Masumoto, N., & Tate, E. W. (2014). Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer. Pancreatology, 14(2), e4.

Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer. / Magee, Anthony I.; Konitsiotis, Antonios D.; Jovanovic, Biljana; Chang, Shu-Chun; Ciepla, Paulina; Masumoto, Naoko; Tate, Edward W.

In: Pancreatology, Vol. 14, No. 2, 2014, p. e4.

Research output: Contribution to journalReview article

Magee, AI, Konitsiotis, AD, Jovanovic, B, Chang, S-C, Ciepla, P, Masumoto, N & Tate, EW 2014, 'Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer', Pancreatology, vol. 14, no. 2, pp. e4.
Magee AI, Konitsiotis AD, Jovanovic B, Chang S-C, Ciepla P, Masumoto N et al. Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer. Pancreatology. 2014;14(2):e4.
Magee, Anthony I. ; Konitsiotis, Antonios D. ; Jovanovic, Biljana ; Chang, Shu-Chun ; Ciepla, Paulina ; Masumoto, Naoko ; Tate, Edward W. / Lipidation of Hedgehog proteins: function, enzymology and therapeutic potential in pancreatic cancer. In: Pancreatology. 2014 ; Vol. 14, No. 2. pp. e4.
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AU - Magee, Anthony I.

AU - Konitsiotis, Antonios D.

AU - Jovanovic, Biljana

AU - Chang, Shu-Chun

AU - Ciepla, Paulina

AU - Masumoto, Naoko

AU - Tate, Edward W.

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N2 - Hedgehog (Hh) proteins (Shh, Dhh and Ihh) are secreted intercellular signalling molecules important in mammalian development and also in the aetiology of many human cancers, hence the Hh signalling pathway is a popular target for development of anti-cancer therapies (1). Hhs are unique in being dually lipidated with a N-terminal amide-linked palmitate and a C-terminal cholesterol, added during its transit through the secretory pathway. These modifications are crucial for assembly into macromolecular complexes for cell-to-cell transport and also for biological function; non-lipidated Hhs are dramatically less potent than the fully lipidated protein.Hedgehog acyltransferase (Hhat) is the enzyme responsible for palmitoylation of the N-terminal cysteine of Hh proteins, and belongs to the membrane bound O-acyltransferase (MBOAT) protein family. Knockdown experiments in pancreatic ductal adenocarcinoma and non-small cell lung cancer cells demonstrate that Hhat is crucial for Shh palmitoylation, assembly into macromolecular complexes and function as assessed by Hh pathway activation, cell growth and invasion, and para/juxtacrine signalling (2). Our results underline the potential of this enzyme as a target for chemotherapy in diverse human cancers including PDAC.Our work has been greatly facilitated by development of bioorthogonal chemical labels for the palmitate and cholesterol moieties (3). Recent results using these to study novel inhibitors will be presented. Hhat is predicted to have 8-12 transmembrane domains but the true number is not known. We have made recent progress in analysis of the membrane topology of Hhat which will allow us better to understand how it functions, and aid in inhibitor screening.1. Dlugosz AA, Talpaz M. Following the hedgehog to new cancer therapies. N Engl J Med 2009; 361: 1202-5.2. Konitsiotis AK, Chang SC, Jovanovic B, Masumoto N, Ciepla P, Palmer CP et al. Attenuation of Hedgehog acyltransferase-catalyzed Sonic hedgehog palmitoylation causes reduced proliferation and invasiveness of human carcinoma cells. PLoSONE in press.3. Heal WP, Jovanovic B, Bessin S, Wright MH, Magee AI and Tate EW. Bioorthogonal chemical tagging of protein cholesterylation in living cells. Chem Comm 2011; 47: 4081-3.

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