Linoleic acid promotes mitochondrial biogenesis and maintains mitochondrial structure for prevention of streptozotocin damage in RIN-m5F cells

Jaan Yeh Jeng, Tian-Shun Tsai, Yi Hua Chiu, Yu Chieh Lee, Hsing-Hsien Cheng, Rong Hong Hsieh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Linoleic acid (LA) improves insulin resistance and prevents diabetes. To investigate whether linoleic acid could protect against streptozotocin (STZ)-induced cell death, rat RIN-m5F cells were exposed to STZ. SL and SO groups consisted of cells treated with STZ and then LA or oleic acid (OA) respectively. STZ treatment decreased the mitochondrial membrane potential in the STZ, SO, and SL groups. Cells of the SL group had more intact mitochondria. Increased mRNA expression of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA), as well as of the mitochondrial biogenesis regulators peroxisome proliferator activated receptor γ coactivator-lα (PGC-lα), and mitochondrial transcription factor A (Tfam), were found in the LA group. The insulin content was significantly decreased in all three groups. These results suggest that the effects of LA on cell viability after STZ damage occur through maintenance of mitochondrial structure and increased mitochondrial biogenesis.

Original languageEnglish
Pages (from-to)1262-1267
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number6
DOIs
Publication statusPublished - 2009

Fingerprint

Organelle Biogenesis
Linoleic Acid
Streptozocin
Mitochondrial DNA
Insulin
Peroxisome Proliferator-Activated Receptors
Mitochondria
Mitochondrial Membrane Potential
Cell death
Oleic Acid
Medical problems
Insulin Resistance
Rats
Cell Survival
Cell Death
Cells
Maintenance
Membranes
Messenger RNA
DNA

Keywords

  • Linoleic acid
  • Mitochondrial biogenesis
  • Mitochondrial transcription factor
  • Oleic acid
  • Streptozotocin

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Linoleic acid promotes mitochondrial biogenesis and maintains mitochondrial structure for prevention of streptozotocin damage in RIN-m5F cells. / Jeng, Jaan Yeh; Tsai, Tian-Shun; Chiu, Yi Hua; Lee, Yu Chieh; Cheng, Hsing-Hsien; Hsieh, Rong Hong.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 6, 2009, p. 1262-1267.

Research output: Contribution to journalArticle

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AU - Lee, Yu Chieh

AU - Cheng, Hsing-Hsien

AU - Hsieh, Rong Hong

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