Line probe assay for rapid detection of mutations in the rpoB gene of Mycobacterium tuberculosis

Yung Ching Liu, Tsi S. Huang, Wen Kuei Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin- susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting.

Original languageEnglish
Pages (from-to)582-585
Number of pages4
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume98
Issue number8
Publication statusPublished - Aug 1999
Externally publishedYes

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Rifampin
Mycobacterium tuberculosis
Mutation
Genes
DNA Sequence Analysis

Keywords

  • INNO-LiPA Rif TB assay
  • Line probe assay
  • Rifampin-resistant Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Line probe assay for rapid detection of mutations in the rpoB gene of Mycobacterium tuberculosis. / Liu, Yung Ching; Huang, Tsi S.; Huang, Wen Kuei.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 98, No. 8, 08.1999, p. 582-585.

Research output: Contribution to journalArticle

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