Abstract
Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin- susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting.
| Original language | English |
|---|---|
| Pages (from-to) | 582-585 |
| Number of pages | 4 |
| Journal | Journal of the Formosan Medical Association = Taiwan yi zhi |
| Volume | 98 |
| Issue number | 8 |
| Publication status | Published - Aug 1999 |
| Externally published | Yes |
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Keywords
- INNO-LiPA Rif TB assay
- Line probe assay
- Rifampin-resistant Mycobacterium tuberculosis
ASJC Scopus subject areas
- Medicine(all)
Cite this
Line probe assay for rapid detection of mutations in the rpoB gene of Mycobacterium tuberculosis. / Liu, Yung Ching; Huang, Tsi S.; Huang, Wen Kuei.
In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 98, No. 8, 08.1999, p. 582-585.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Line probe assay for rapid detection of mutations in the rpoB gene of Mycobacterium tuberculosis
AU - Liu, Yung Ching
AU - Huang, Tsi S.
AU - Huang, Wen Kuei
PY - 1999/8
Y1 - 1999/8
N2 - Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin- susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting.
AB - Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin- susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting.
KW - INNO-LiPA Rif TB assay
KW - Line probe assay
KW - Rifampin-resistant Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=0032849566&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032849566&partnerID=8YFLogxK
M3 - Article
C2 - 10502914
AN - SCOPUS:0032849566
VL - 98
SP - 582
EP - 585
JO - Journal of the Formosan Medical Association
JF - Journal of the Formosan Medical Association
SN - 0929-6646
IS - 8
ER -