Ligusticum chuanxiong as a potential neuroprotectant for preventing serum deprivation-induced apoptosis in rat pheochromocytoma cells

Functional roles of mitogen-activated protein kinases

Yun Lian Lin, Guei Jane Wang, Chuen Lin Huang, Yi Chao Lee, Wei Chen Liao, Wen Lin Lai, Yen Ju Lin, Nai Kuei Huang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Ligusticum chuanxiong (LC) as a common component in many traditional Chinese medicinal formulas and decoctions has been used to treat different central nervous diseases, suggesting a neuroprotective function. Aim of the study: To investigate the functional roles of mitogen-activated protein kinases (MAPKs) in mediating the neuroprotection of LC. Materials and methods: Different extractions of LC were applied with or without MAPK inhibitor to test their protection against serum deprivation-induced apoptosis in rat neuronal-like pheochromocytoma (PC12) cells as revealed by an MTT assay or Hoechst staining. Western blot was used to identify the activations of MAPKs. Results: The most effective butanol extraction (LC-BuOH) was used in the following experiments. LC-BuOH reversed serum deprivation-induced decreased phosphorylation of extracellular signal-regulated kinase (ERK) and increased phosphorylation of c-Jun NH2-terminal kinase (JNK) and p38, the family of MAPKs. A PKA inhibitor, blocked the protection of LC-BuOH and partially blocked LC-BuOH-induced alterations in MAPKs, suggesting that the LC-BuOH regulates MAPKs through both PKA-dependent and -independent pathways. Although PD 98059, an inhibitor of MEK which activates ERK, blocked LC-BuOH-induced ERK phosphorylation, it did not block the protection of LC-BuOH. Conclusions: LC-BuOH mediates protection by suppressing JNK/p38 instead of activating ERK activity.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalJournal of Ethnopharmacology
Volume122
Issue number3
DOIs
Publication statusPublished - Apr 21 2009
Externally publishedYes

Fingerprint

Ligusticum
Pheochromocytoma
Neuroprotective Agents
Mitogen-Activated Protein Kinases
Apoptosis
Serum
Extracellular Signal-Regulated MAP Kinases
JNK Mitogen-Activated Protein Kinases
Phosphorylation
Butanols
PC12 Cells
Mitogen-Activated Protein Kinase Kinases
p38 Mitogen-Activated Protein Kinases
Protein Kinase Inhibitors

Keywords

  • Apoptosis
  • Ligusticum chuanxiong
  • MAPKs
  • Pheochromocytoma cells
  • Serum deprivation

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Ligusticum chuanxiong as a potential neuroprotectant for preventing serum deprivation-induced apoptosis in rat pheochromocytoma cells : Functional roles of mitogen-activated protein kinases. / Lin, Yun Lian; Wang, Guei Jane; Huang, Chuen Lin; Lee, Yi Chao; Liao, Wei Chen; Lai, Wen Lin; Lin, Yen Ju; Huang, Nai Kuei.

In: Journal of Ethnopharmacology, Vol. 122, No. 3, 21.04.2009, p. 417-423.

Research output: Contribution to journalArticle

Lin, Yun Lian ; Wang, Guei Jane ; Huang, Chuen Lin ; Lee, Yi Chao ; Liao, Wei Chen ; Lai, Wen Lin ; Lin, Yen Ju ; Huang, Nai Kuei. / Ligusticum chuanxiong as a potential neuroprotectant for preventing serum deprivation-induced apoptosis in rat pheochromocytoma cells : Functional roles of mitogen-activated protein kinases. In: Journal of Ethnopharmacology. 2009 ; Vol. 122, No. 3. pp. 417-423.
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abstract = "Ethnopharmacological relevance: Ligusticum chuanxiong (LC) as a common component in many traditional Chinese medicinal formulas and decoctions has been used to treat different central nervous diseases, suggesting a neuroprotective function. Aim of the study: To investigate the functional roles of mitogen-activated protein kinases (MAPKs) in mediating the neuroprotection of LC. Materials and methods: Different extractions of LC were applied with or without MAPK inhibitor to test their protection against serum deprivation-induced apoptosis in rat neuronal-like pheochromocytoma (PC12) cells as revealed by an MTT assay or Hoechst staining. Western blot was used to identify the activations of MAPKs. Results: The most effective butanol extraction (LC-BuOH) was used in the following experiments. LC-BuOH reversed serum deprivation-induced decreased phosphorylation of extracellular signal-regulated kinase (ERK) and increased phosphorylation of c-Jun NH2-terminal kinase (JNK) and p38, the family of MAPKs. A PKA inhibitor, blocked the protection of LC-BuOH and partially blocked LC-BuOH-induced alterations in MAPKs, suggesting that the LC-BuOH regulates MAPKs through both PKA-dependent and -independent pathways. Although PD 98059, an inhibitor of MEK which activates ERK, blocked LC-BuOH-induced ERK phosphorylation, it did not block the protection of LC-BuOH. Conclusions: LC-BuOH mediates protection by suppressing JNK/p38 instead of activating ERK activity.",
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AU - Wang, Guei Jane

AU - Huang, Chuen Lin

AU - Lee, Yi Chao

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AU - Lai, Wen Lin

AU - Lin, Yen Ju

AU - Huang, Nai Kuei

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