Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery

Erh Hsuan Lin, Michelle Keramidas, Claire Rome, Wen Ta Chiu, Cheng Wen Wu, Jean Luc Coll, Win Ping Deng

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Polyethyleneimine (PEI) is a cationic polymer that is effective in gene delivery in vivo. Plasmid DNA incorporating the Sleeping-Beauty (SB) transposon has been shown to induce long-term transgene expression in mouse lungs after PEI-mediated delivery. In the current report, we followed the reporter gene expression mediated by PEI/SB delivery in lungs of mice using the non-invasive bioluminescent imaging (BLI) technology. After delivery, the reporter gene signal showed a rapid decay in the first two weeks to a nearly undetectable level, but then the signal augmented gradually in the following weeks and finally reached a stable level that maintained until the natural death of animals. The stabilization of transgene expression is associated with the multiplication of a small number of PEI/SB-labeled alveolar cells, which proliferated both under normal conditions and in response to acute local injury for epithelia repair, and may play a role in long-term homeostatic maintenance in alveoli. The data presented here suggests that systemic delivery of PEI/SB induces stable transfection specifically in a small population of alveolar progenitor cells. The technique provides a promising platform for future research in distal lung biology and tissue regenerative therapy.

Original languageEnglish
Pages (from-to)1978-1985
Number of pages8
JournalBiomaterials
Volume32
Issue number7
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Alveolar epithelial cells (AECs)
  • Non-invasive bioluminescent imaging (BLI)
  • Polyethyleneimine (PEI)
  • Sleeping-Beauty (SB)

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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