Licochalcone A inhibits the migration and invasion of human lung cancer cells via inactivation of the Akt signaling pathway with downregulation of MMP-1/-3 expression

Hung Che Huang, Lo Lin Tsai, Jen Pi Tsai, Shu Ching Hsieh, Shun Fa Yang, Jung Tsung Hsueh, Yi Hsien Hsieh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Licochalcone A (LicA), a major phenolic constituent of Glycyrrhiza inflata, has been reported to exhibit anti-tumor, anti-inflammatory, and anti-metastatic properties in various cancer cells and animal models. The aim of this study was to determine the anti-tumor effects of LicA on lung cancer cells. The results indicated that LicA exhibited effective inhibition of cell migration and invasion of A549 and H460 cells under non-cytotoxic concentrations. Furthermore, LicA was also found to significantly inhibit the proteins and messenger RNA (mRNA) expression of MMP-1 and MMP-3 in A549 cells. Moreover, treatment of A549 cells with LicA-inhibited activation of the phosphorylation of Akt and inhibition of Akt by LY294002 (PI3K inhibitor) or transfection with the constitutive active-Akt (CA-Akt) expression vector significantly abolished the LicA-inhibited migration and invasion through activation of the Akt pathway. Further mechanistic studies revealed that LicA inhibits Akt signaling pathways and downstream transcription factors Sp1 expression. These findings imply a critical role for Akt inhibition in the LicA-inhibited migration and invasion of lung cancer cells. Thus, LicA might be used as an anti-invasive agent in the treatment of lung cancer.

Original languageEnglish
JournalTumor Biology
DOIs
Publication statusAccepted/In press - Aug 23 2014
Externally publishedYes

Keywords

  • Invasion
  • LicA
  • Lung cancer cells
  • Migration
  • MMP-1
  • MMP-3
  • Sp1

ASJC Scopus subject areas

  • Medicine(all)
  • Cancer Research

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