Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass: clinical correlations.

Chi Woon Kong, Cheng Hsiung Huang, Tai Ger Hsu, Kelvin K C Tsai, Chen Fu Hsu, Mei Chun Huang, Ching Lung Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cardiac surgery with the use of cardiopulmonary bypass (CPB) is known to initiate systemic inflammatory responses that are associated with immune dysregulations, but the pathomechanisms underlying these changes remain elusive. Mitochondrial transmembrane potential (MTP) is an important determinant of leukocytic functions and viability, and may be altered as a part of the cellular responses to systemic inflammatory insults. Therefore, we examined MTP in three subsets of peripheral leukocytes in 18 patients receiving uncomplicated cardiac surgery involving CPB. The MTP of neutrophils and lymphocytes significantly increased, whereas that of monocytes significantly declined, after the surgery. The alterations in leukocytic MTP were transient, normalizing 3 days to 1 week after the surgery, and were accompanied by transient overproduction of intracellular oxidants, including nitric oxide and superoxide. Despite these perturbations, the viability status of leukocytes remained unaltered. Positive correlations were found between the changes of leukocyte MTP and various clinical parameters, implying that leukocyte mitochondrial alterations are parts of the systemic immune perturbations induced by the bypass surgery.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalShock
Volume21
Issue number4
Publication statusPublished - Apr 2004
Externally publishedYes

Fingerprint

Cardiopulmonary Bypass
Membrane Potentials
Thoracic Surgery
Leukocytes
Oxidants
Superoxides
Monocytes
Nitric Oxide
Neutrophils
Lymphocytes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Kong, C. W., Huang, C. H., Hsu, T. G., Tsai, K. K. C., Hsu, C. F., Huang, M. C., & Chen, C. L. (2004). Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass: clinical correlations. Shock, 21(4), 315-319.

Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass : clinical correlations. / Kong, Chi Woon; Huang, Cheng Hsiung; Hsu, Tai Ger; Tsai, Kelvin K C; Hsu, Chen Fu; Huang, Mei Chun; Chen, Ching Lung.

In: Shock, Vol. 21, No. 4, 04.2004, p. 315-319.

Research output: Contribution to journalArticle

Kong, CW, Huang, CH, Hsu, TG, Tsai, KKC, Hsu, CF, Huang, MC & Chen, CL 2004, 'Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass: clinical correlations.', Shock, vol. 21, no. 4, pp. 315-319.
Kong, Chi Woon ; Huang, Cheng Hsiung ; Hsu, Tai Ger ; Tsai, Kelvin K C ; Hsu, Chen Fu ; Huang, Mei Chun ; Chen, Ching Lung. / Leukocyte mitochondrial alterations after cardiac surgery involving cardiopulmonary bypass : clinical correlations. In: Shock. 2004 ; Vol. 21, No. 4. pp. 315-319.
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