Let-7c restores radiosensitivity and chemosensitivity and impairs stemness in oral cancer cells through inhibiting interleukin-8

Chih Yu Peng, Tung Yuan Wang, Shiuan Shinn Lee, Pei Ling Hsieh, Yi Wen Liao, Lo Lin Tsai, Chih Yuan Fang, Cheng Chia Yu, Ching Shui Hsieh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The let-7 family of microRNAs has been considered as tumor suppressors in various cancers; however, the role of let-7c in oral squamous cell carcinoma has not been determined yet. Methods: In this study, phenotypical behaviors and the radio/chemoresistance were examined subsequent to overexpression of let-7c. In addition, the expression of let-7c in cancer stem cells (CSCs) was evaluated and the effect of let-7c on stemness characteristics was assessed. Also, luciferase activity assays were performed to test whether interleukin (IL)-8 was a putative target of let-7c. Results: Our results confirmed that the expression of let-7c in CSCs was reduced, while overexpression of let-7c attenuated the oncogenicity. Moreover, ectopic expression of let-7c in CSCs downregulated the stemness hallmarks and the radio/chemoresistance. Expression and secretion of IL-8 in oral CSCs were both reduced following overexpression of let-7c. Besides, the inhibitory effect of let-7c on various stemness phenotypes was reverted by IL-8, indicating that lower expression of let-7c may confer higher cancer stemness through a failure to downregulate IL-8. Conclusion: These findings revealed the significance of let-7c in the contribution of oral cancer stemness and radio/chemoresistance. Targeting let-7c and its downstream IL-8 may be beneficial to prevent cancer recurrence and metastasis of oral squamous cell carcinoma.

Original languageEnglish
JournalJournal of Oral Pathology and Medicine
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Mouth Neoplasms
Radiation Tolerance
Interleukin-8
Neoplastic Stem Cells
Radio
Squamous Cell Carcinoma
Neoplasms
Down-Regulation
Luciferases
MicroRNAs
Neoplasm Metastasis
Phenotype
Recurrence

Keywords

  • Cancer stemness
  • Interleukin-8
  • Let-7c
  • Oral squamous cell carcinomas
  • Radio/chemoresistance

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Let-7c restores radiosensitivity and chemosensitivity and impairs stemness in oral cancer cells through inhibiting interleukin-8. / Peng, Chih Yu; Wang, Tung Yuan; Lee, Shiuan Shinn; Hsieh, Pei Ling; Liao, Yi Wen; Tsai, Lo Lin; Fang, Chih Yuan; Yu, Cheng Chia; Hsieh, Ching Shui.

In: Journal of Oral Pathology and Medicine, 01.01.2018.

Research output: Contribution to journalArticle

Peng, Chih Yu ; Wang, Tung Yuan ; Lee, Shiuan Shinn ; Hsieh, Pei Ling ; Liao, Yi Wen ; Tsai, Lo Lin ; Fang, Chih Yuan ; Yu, Cheng Chia ; Hsieh, Ching Shui. / Let-7c restores radiosensitivity and chemosensitivity and impairs stemness in oral cancer cells through inhibiting interleukin-8. In: Journal of Oral Pathology and Medicine. 2018.
@article{08d8ada61bb24f4aa067c49155928b1d,
title = "Let-7c restores radiosensitivity and chemosensitivity and impairs stemness in oral cancer cells through inhibiting interleukin-8",
abstract = "Background: The let-7 family of microRNAs has been considered as tumor suppressors in various cancers; however, the role of let-7c in oral squamous cell carcinoma has not been determined yet. Methods: In this study, phenotypical behaviors and the radio/chemoresistance were examined subsequent to overexpression of let-7c. In addition, the expression of let-7c in cancer stem cells (CSCs) was evaluated and the effect of let-7c on stemness characteristics was assessed. Also, luciferase activity assays were performed to test whether interleukin (IL)-8 was a putative target of let-7c. Results: Our results confirmed that the expression of let-7c in CSCs was reduced, while overexpression of let-7c attenuated the oncogenicity. Moreover, ectopic expression of let-7c in CSCs downregulated the stemness hallmarks and the radio/chemoresistance. Expression and secretion of IL-8 in oral CSCs were both reduced following overexpression of let-7c. Besides, the inhibitory effect of let-7c on various stemness phenotypes was reverted by IL-8, indicating that lower expression of let-7c may confer higher cancer stemness through a failure to downregulate IL-8. Conclusion: These findings revealed the significance of let-7c in the contribution of oral cancer stemness and radio/chemoresistance. Targeting let-7c and its downstream IL-8 may be beneficial to prevent cancer recurrence and metastasis of oral squamous cell carcinoma.",
keywords = "Cancer stemness, Interleukin-8, Let-7c, Oral squamous cell carcinomas, Radio/chemoresistance",
author = "Peng, {Chih Yu} and Wang, {Tung Yuan} and Lee, {Shiuan Shinn} and Hsieh, {Pei Ling} and Liao, {Yi Wen} and Tsai, {Lo Lin} and Fang, {Chih Yuan} and Yu, {Cheng Chia} and Hsieh, {Ching Shui}",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/jop.12711",
language = "English",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Let-7c restores radiosensitivity and chemosensitivity and impairs stemness in oral cancer cells through inhibiting interleukin-8

AU - Peng, Chih Yu

AU - Wang, Tung Yuan

AU - Lee, Shiuan Shinn

AU - Hsieh, Pei Ling

AU - Liao, Yi Wen

AU - Tsai, Lo Lin

AU - Fang, Chih Yuan

AU - Yu, Cheng Chia

AU - Hsieh, Ching Shui

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: The let-7 family of microRNAs has been considered as tumor suppressors in various cancers; however, the role of let-7c in oral squamous cell carcinoma has not been determined yet. Methods: In this study, phenotypical behaviors and the radio/chemoresistance were examined subsequent to overexpression of let-7c. In addition, the expression of let-7c in cancer stem cells (CSCs) was evaluated and the effect of let-7c on stemness characteristics was assessed. Also, luciferase activity assays were performed to test whether interleukin (IL)-8 was a putative target of let-7c. Results: Our results confirmed that the expression of let-7c in CSCs was reduced, while overexpression of let-7c attenuated the oncogenicity. Moreover, ectopic expression of let-7c in CSCs downregulated the stemness hallmarks and the radio/chemoresistance. Expression and secretion of IL-8 in oral CSCs were both reduced following overexpression of let-7c. Besides, the inhibitory effect of let-7c on various stemness phenotypes was reverted by IL-8, indicating that lower expression of let-7c may confer higher cancer stemness through a failure to downregulate IL-8. Conclusion: These findings revealed the significance of let-7c in the contribution of oral cancer stemness and radio/chemoresistance. Targeting let-7c and its downstream IL-8 may be beneficial to prevent cancer recurrence and metastasis of oral squamous cell carcinoma.

AB - Background: The let-7 family of microRNAs has been considered as tumor suppressors in various cancers; however, the role of let-7c in oral squamous cell carcinoma has not been determined yet. Methods: In this study, phenotypical behaviors and the radio/chemoresistance were examined subsequent to overexpression of let-7c. In addition, the expression of let-7c in cancer stem cells (CSCs) was evaluated and the effect of let-7c on stemness characteristics was assessed. Also, luciferase activity assays were performed to test whether interleukin (IL)-8 was a putative target of let-7c. Results: Our results confirmed that the expression of let-7c in CSCs was reduced, while overexpression of let-7c attenuated the oncogenicity. Moreover, ectopic expression of let-7c in CSCs downregulated the stemness hallmarks and the radio/chemoresistance. Expression and secretion of IL-8 in oral CSCs were both reduced following overexpression of let-7c. Besides, the inhibitory effect of let-7c on various stemness phenotypes was reverted by IL-8, indicating that lower expression of let-7c may confer higher cancer stemness through a failure to downregulate IL-8. Conclusion: These findings revealed the significance of let-7c in the contribution of oral cancer stemness and radio/chemoresistance. Targeting let-7c and its downstream IL-8 may be beneficial to prevent cancer recurrence and metastasis of oral squamous cell carcinoma.

KW - Cancer stemness

KW - Interleukin-8

KW - Let-7c

KW - Oral squamous cell carcinomas

KW - Radio/chemoresistance

UR - http://www.scopus.com/inward/record.url?scp=85045752328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045752328&partnerID=8YFLogxK

U2 - 10.1111/jop.12711

DO - 10.1111/jop.12711

M3 - Article

AN - SCOPUS:85045752328

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

ER -