Leaf extracts of calocedrus formosana (Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells

Chen Li Cheng, Sheau Yun Yuan, Chi Chen Lin, Shih Lan Hsu, Ya Wen Cheng, Jyh Horng Wu, Chi Rei Yang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Calocedrus formosana (Florin) bark acetone/ethylacetate extracts are known to exert an antitumor effect on some human cancer cell lines, but the mechanism is yet to be defined. The aim of this study was to determine the effects of Florin leaf methanol extracts on the growth and apoptosis of human bladder cancer cell lines. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that the growth of these bladder cancer cells was potently inhibited by the Florin leaf extracts. The cell cycle of these extract-treated cells (TCCSUP cells) was arrested at the G2/M phase as determined by flow cytometry. Western blot analysis revealed the increases of cyclin B1 and Cdc2 kinase levels, alone with the decrease of phosphorylated Cdc2 kinase, after treating these cells with the extracts. An immunofluorescence assessment of -tubulin showed decreased levels of polymerized tubulin in treated cells. However, the proteolytic cleavage of poly ADP-ribose polymerase and the activation of caspase-3/-8/-9 were all increased upon treatments of extracts. The concurrent increase of Bax and decrease of Bcl-2 levels indicated that the extracts could induce apoptosis in these treated cells. Taken together, these results suggest that the Florin leaf extracts may be an effective antibladder cancer agent.

Original languageEnglish
Article number380923
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011
Externally publishedYes

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Cupressaceae
G2 Phase Cell Cycle Checkpoints
Urinary Bladder Neoplasms
Apoptosis
Tubulin
Cell Extracts
Phosphotransferases
Cyclin B1
Cell Line
Poly(ADP-ribose) Polymerases
Caspase 8
G2 Phase
Growth
Acetone
Caspase 3
Cell Division
Fluorescent Antibody Technique
Methanol
Neoplasms
Cell Cycle

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Leaf extracts of calocedrus formosana (Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells. / Cheng, Chen Li; Yuan, Sheau Yun; Lin, Chi Chen; Hsu, Shih Lan; Cheng, Ya Wen; Wu, Jyh Horng; Yang, Chi Rei.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2011, 380923, 2011.

Research output: Contribution to journalArticle

Cheng, Chen Li ; Yuan, Sheau Yun ; Lin, Chi Chen ; Hsu, Shih Lan ; Cheng, Ya Wen ; Wu, Jyh Horng ; Yang, Chi Rei. / Leaf extracts of calocedrus formosana (Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells. In: Evidence-based Complementary and Alternative Medicine. 2011 ; Vol. 2011.
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