Layer-by-layer thin film of reduced graphene oxide and gold nanoparticles as an effective sample plate in laser-induced desorption/ionization mass spectrometry

Tsung Rong Kuo, Di Yan Wang, Yu Chen Chiu, Yun Chieh Yeh, Wei Ting Chen, Ching Hui Chen, Chun Wei Chen, Huan Cheng Chang, Cho Chun Hu, Chia Chun Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This work demonstrated a simple platform for rapid and effective surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) measurements based on the layer structure of reduced graphene oxide (rGO) and gold nanoparticles. A multi-layer thin film was fabricated by alternate layer-by-layer depositions of rGO and gold nanoparticles (LBL rGO/AuNP). The flat and clean two-dimensional film was served as the sample plate and also functioned as the matrix in SALDI-TOF MS. By simply one-step deposition of analytes onto the LBL rGO/AuNP sample plate, the MS measurements of various homogeneous samples were ready to execute. The optimization of MS signal was reached by the variation of the layer numbers of rGO and gold nanoparticles. Also, the small molecules including amino acids, carbohydrates and peptides were successfully analyzed in SALDI-TOF MS using the LBL rGO/AuNP sample plate. The results showed that the signal intensity, SN-1 ratio and reproducibility of SALDI-TOF spectra have been significantly improved in comparison to the uses of gold nanoparticles or α-cyano-4-hydroxy-cinnamic acid (CHCA) as the assisted matrixes. Taking the advantages of the unique properties of rGO and gold nanoparticles, the ready-to-use MS sample plate, which could absorb and dissipate laser energy to analytes quite efficiently and homogeneously, has shown great commercial potentials for MS applications.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalAnalytica Chimica Acta
Volume809
DOIs
Publication statusPublished - Jan 27 2014
Externally publishedYes

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Graphite
Gold
Nanoparticles
Oxides
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
desorption
Lasers
ionization
gold
mass spectrometry
laser
oxide
Thin films
Hydroxy Acids
matrix
nanoparticle
peptide

Keywords

  • Gold nanoparticles
  • Laser-induced desorption/ionization mass spectrometry
  • Multi-layer thin film
  • Reduced graphene oxide
  • Sample plate

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry
  • Medicine(all)

Cite this

Layer-by-layer thin film of reduced graphene oxide and gold nanoparticles as an effective sample plate in laser-induced desorption/ionization mass spectrometry. / Kuo, Tsung Rong; Wang, Di Yan; Chiu, Yu Chen; Yeh, Yun Chieh; Chen, Wei Ting; Chen, Ching Hui; Chen, Chun Wei; Chang, Huan Cheng; Hu, Cho Chun; Chen, Chia Chun.

In: Analytica Chimica Acta, Vol. 809, 27.01.2014, p. 97-103.

Research output: Contribution to journalArticle

Kuo, Tsung Rong ; Wang, Di Yan ; Chiu, Yu Chen ; Yeh, Yun Chieh ; Chen, Wei Ting ; Chen, Ching Hui ; Chen, Chun Wei ; Chang, Huan Cheng ; Hu, Cho Chun ; Chen, Chia Chun. / Layer-by-layer thin film of reduced graphene oxide and gold nanoparticles as an effective sample plate in laser-induced desorption/ionization mass spectrometry. In: Analytica Chimica Acta. 2014 ; Vol. 809. pp. 97-103.
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AU - Wang, Di Yan

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AU - Yeh, Yun Chieh

AU - Chen, Wei Ting

AU - Chen, Ching Hui

AU - Chen, Chun Wei

AU - Chang, Huan Cheng

AU - Hu, Cho Chun

AU - Chen, Chia Chun

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AB - This work demonstrated a simple platform for rapid and effective surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) measurements based on the layer structure of reduced graphene oxide (rGO) and gold nanoparticles. A multi-layer thin film was fabricated by alternate layer-by-layer depositions of rGO and gold nanoparticles (LBL rGO/AuNP). The flat and clean two-dimensional film was served as the sample plate and also functioned as the matrix in SALDI-TOF MS. By simply one-step deposition of analytes onto the LBL rGO/AuNP sample plate, the MS measurements of various homogeneous samples were ready to execute. The optimization of MS signal was reached by the variation of the layer numbers of rGO and gold nanoparticles. Also, the small molecules including amino acids, carbohydrates and peptides were successfully analyzed in SALDI-TOF MS using the LBL rGO/AuNP sample plate. The results showed that the signal intensity, SN-1 ratio and reproducibility of SALDI-TOF spectra have been significantly improved in comparison to the uses of gold nanoparticles or α-cyano-4-hydroxy-cinnamic acid (CHCA) as the assisted matrixes. Taking the advantages of the unique properties of rGO and gold nanoparticles, the ready-to-use MS sample plate, which could absorb and dissipate laser energy to analytes quite efficiently and homogeneously, has shown great commercial potentials for MS applications.

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