Laryngeal C-fiber afferents are not involved in the apneic response to laryngeal wood smoke in anesthetized rats

You Shuei Lin, Ching Yin Ho, Shyue Yih Chang, Yu Ru Kou

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Laryngeal exposure to wood smoke in rats evokes a reflex apnea which is mediated through superior laryngeal afferents (J. Appl. Physiol. 83: 723-730, 1997). To study the role of laryngeal C-fiber afferents in eliciting this response, capsaicin aerosol (0.05 - 0.2 μg/ml) and 5 ml of wood smoke were delivered separately into a functionally isolated larynx of anesthetized Sprague-Dawley rats at a constant flow rate of 1.4 ml/s, while animals breathed spontaneously. Studies were repeated after either an intravenous injection of ruthenium red (2 mg/kg; n = 8), a perineural capsaicin treatment (200 μg/ml for 5 min; n = 8) of the superior laryngeal nerves, or a perineural sham treatment (n = 8); Ruthenium red inhibits the stimulation of afferent C-fiber nerve endings by capsaicin, whereas perineural capsaicin treatment selective blocks the conduction of C-fiber afferents. Either ruthenium red or perineural capsaicin treatment abolished the apneic response to laryngeal capsaicin, but did not significantly affect the apneic response to laryngeal wood smoke. Furthermore, the apneic responses to both types of irritants were not significantly altered by perineural sham treatment, yet were completely eliminated by a subsequent denervation of superior laryngeal nerves. Our results suggest that superior laryngeal C-fiber afferents are not involved in eliciting the reflex apneic response to laryngeal wood smoke in anesthetized rats. It is speculated that this response may result mainly from the stimulation of myelinated afferents, possibly laryngeal irritant receptors.

Original languageEnglish
Pages (from-to)1695-1704
Number of pages10
JournalLife Sciences
Volume66
Issue number18
DOIs
Publication statusPublished - Mar 24 2000
Externally publishedYes

Fingerprint

Unmyelinated Nerve Fibers
Capsaicin
Smoke
Rats
Wood
Ruthenium Red
Fibers
Laryngeal Nerves
Irritants
Reflex
Placebos
Nerve Endings
Denervation
Apnea
Larynx
Aerosols
Intravenous Injections
Sprague Dawley Rats
Animals
Therapeutics

Keywords

  • Inhaled irritants
  • Perineural capsaicin treatment
  • Ruthenium red
  • Smoke
  • Superior laryngeal nerves

ASJC Scopus subject areas

  • Pharmacology

Cite this

Laryngeal C-fiber afferents are not involved in the apneic response to laryngeal wood smoke in anesthetized rats. / Lin, You Shuei; Ho, Ching Yin; Chang, Shyue Yih; Kou, Yu Ru.

In: Life Sciences, Vol. 66, No. 18, 24.03.2000, p. 1695-1704.

Research output: Contribution to journalArticle

Lin, You Shuei ; Ho, Ching Yin ; Chang, Shyue Yih ; Kou, Yu Ru. / Laryngeal C-fiber afferents are not involved in the apneic response to laryngeal wood smoke in anesthetized rats. In: Life Sciences. 2000 ; Vol. 66, No. 18. pp. 1695-1704.
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