Large scale chromatographic purification of alpha 1-antitrypsin from human plasma supernatant II + III.

T. Burnouf, J. Constans, A. Clerc, J. Descamps, L. Martinache, M. Goudemand

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Abstract

We describe a large-scale chromatographic method to purify alpha 1-antitrypsin (AAT) from human plasma supernatant II + III. Supernatant II + III was injected onto DEAE sepharose CL 6B to get an albumin-rich fraction as well as a fraction that contained about 45% AAT. This intermediate purity AAT fraction was further purified by size-exclusion chromatography on Sephacryl S-200. The product was heated in solution at 60 degrees C for 10 hours in the presence of a stabilizer to lower the risk of transmission of viral diseases. Purity of the AAT concentrate is over 85%; clinical testing of its safety and efficacy in the treatment of PiZ patients is underway.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalDevelopments in Biological Standardization
Volume67
Publication statusPublished - 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology

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