Langerhans cells in 60 odontogenic keratocysts

Chun Han Chang, Yang Che Wu, Yu Hsueh Wu, Andy Sun, Hsin Ming Chen, Hung Pin Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background/purpose Langerhans cells (LCs) are antigen-presenting cells. This study mainly evaluated the LC counts in 60 odontogenic keratocysts (OKCs). Materials and methods The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 60 OKC sites without inflammation, 39 OKC sites with mild/moderate inflammation, and 13 OKC sites with severe inflammation from 60 OKC specimens. Results The mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.4 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (5.3 ± 2.5 and 2.5 ± 2.7 cells/HPF, respectively) to severe inflammation OKC sites (12.7 ± 5.6 and 9.3 ± 7.2 cells/HPF, respectively; all P-values < 0.001). OKC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues of OKCs were significantly higher in the thicker lining epithelium (>100 μm) group (6.8 ± 5.1 and 3.7 ± 4.9 cells/HPF, respectively) than in the thinner lining epithelium (≦100 μm) group (1.0 ± 1.7 and 0.8 ± 2.5 cell/HPF, respectively; both P-values < 0.001). Conclusion There is a significant association of inflammation grade with the number of LCs in OKCs. The scarce LCs in the lining epithelia of OKCs without inflammation suggests the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalJournal of Dental Sciences
Volume12
Issue number3
DOIs
Publication statusPublished - Sep 1 2017
Externally publishedYes

Fingerprint

Odontogenic Cysts
Langerhans Cells
Inflammation
Epithelium
Cell Count
Connective Tissue
Immunologic Monitoring
Antigen-Presenting Cells
Epithelial Cells

Keywords

  • aggressive clinical behavior
  • CD1a
  • high recurrence rate
  • immunosurveillance
  • Langerhans cell
  • odontogenic keratocyst

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Chang, C. H., Wu, Y. C., Wu, Y. H., Sun, A., Chen, H. M., & Lin, H. P. (2017). Langerhans cells in 60 odontogenic keratocysts. Journal of Dental Sciences, 12(3), 283-290. https://doi.org/10.1016/j.jds.2017.04.001

Langerhans cells in 60 odontogenic keratocysts. / Chang, Chun Han; Wu, Yang Che; Wu, Yu Hsueh; Sun, Andy; Chen, Hsin Ming; Lin, Hung Pin.

In: Journal of Dental Sciences, Vol. 12, No. 3, 01.09.2017, p. 283-290.

Research output: Contribution to journalArticle

Chang, CH, Wu, YC, Wu, YH, Sun, A, Chen, HM & Lin, HP 2017, 'Langerhans cells in 60 odontogenic keratocysts', Journal of Dental Sciences, vol. 12, no. 3, pp. 283-290. https://doi.org/10.1016/j.jds.2017.04.001
Chang, Chun Han ; Wu, Yang Che ; Wu, Yu Hsueh ; Sun, Andy ; Chen, Hsin Ming ; Lin, Hung Pin. / Langerhans cells in 60 odontogenic keratocysts. In: Journal of Dental Sciences. 2017 ; Vol. 12, No. 3. pp. 283-290.
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abstract = "Background/purpose Langerhans cells (LCs) are antigen-presenting cells. This study mainly evaluated the LC counts in 60 odontogenic keratocysts (OKCs). Materials and methods The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 60 OKC sites without inflammation, 39 OKC sites with mild/moderate inflammation, and 13 OKC sites with severe inflammation from 60 OKC specimens. Results The mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.4 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (5.3 ± 2.5 and 2.5 ± 2.7 cells/HPF, respectively) to severe inflammation OKC sites (12.7 ± 5.6 and 9.3 ± 7.2 cells/HPF, respectively; all P-values < 0.001). OKC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues of OKCs were significantly higher in the thicker lining epithelium (>100 μm) group (6.8 ± 5.1 and 3.7 ± 4.9 cells/HPF, respectively) than in the thinner lining epithelium (≦100 μm) group (1.0 ± 1.7 and 0.8 ± 2.5 cell/HPF, respectively; both P-values < 0.001). Conclusion There is a significant association of inflammation grade with the number of LCs in OKCs. The scarce LCs in the lining epithelia of OKCs without inflammation suggests the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.",
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N2 - Background/purpose Langerhans cells (LCs) are antigen-presenting cells. This study mainly evaluated the LC counts in 60 odontogenic keratocysts (OKCs). Materials and methods The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 60 OKC sites without inflammation, 39 OKC sites with mild/moderate inflammation, and 13 OKC sites with severe inflammation from 60 OKC specimens. Results The mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.4 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (5.3 ± 2.5 and 2.5 ± 2.7 cells/HPF, respectively) to severe inflammation OKC sites (12.7 ± 5.6 and 9.3 ± 7.2 cells/HPF, respectively; all P-values < 0.001). OKC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues of OKCs were significantly higher in the thicker lining epithelium (>100 μm) group (6.8 ± 5.1 and 3.7 ± 4.9 cells/HPF, respectively) than in the thinner lining epithelium (≦100 μm) group (1.0 ± 1.7 and 0.8 ± 2.5 cell/HPF, respectively; both P-values < 0.001). Conclusion There is a significant association of inflammation grade with the number of LCs in OKCs. The scarce LCs in the lining epithelia of OKCs without inflammation suggests the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.

AB - Background/purpose Langerhans cells (LCs) are antigen-presenting cells. This study mainly evaluated the LC counts in 60 odontogenic keratocysts (OKCs). Materials and methods The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 60 OKC sites without inflammation, 39 OKC sites with mild/moderate inflammation, and 13 OKC sites with severe inflammation from 60 OKC specimens. Results The mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.4 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (5.3 ± 2.5 and 2.5 ± 2.7 cells/HPF, respectively) to severe inflammation OKC sites (12.7 ± 5.6 and 9.3 ± 7.2 cells/HPF, respectively; all P-values < 0.001). OKC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues of OKCs were significantly higher in the thicker lining epithelium (>100 μm) group (6.8 ± 5.1 and 3.7 ± 4.9 cells/HPF, respectively) than in the thinner lining epithelium (≦100 μm) group (1.0 ± 1.7 and 0.8 ± 2.5 cell/HPF, respectively; both P-values < 0.001). Conclusion There is a significant association of inflammation grade with the number of LCs in OKCs. The scarce LCs in the lining epithelia of OKCs without inflammation suggests the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.

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KW - immunosurveillance

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KW - odontogenic keratocyst

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