Lactoferrin protects against chemical-induced rat liver fibrosis by inhibiting stellate cell activation

Yu Tang Tung, Ting Yu Tang, Hsiao Ling Chen, Shang Hsun Yang, Kowit Yu Chong, Winston T K Cheng, Chuan Mu Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Liver diseases, which can be caused by alcohol abuse, chemical intoxication, viral hepatitis infection, and autoimmune disorders, are a significant health issue because they can develop into liver fibrosis and cirrhosis. Lactoferrin (LF), a siderophilic protein with 2 iron-binding sites, has been demonstrated to possess a multitude of biological functions, including antiinflammation, anticancer, and antimicrobial effects, as well as immunomodulatory-enhancing functions. In the current study, we induced hepatotoxicity in rats with dimethylnitrosamine (DMN) to establish a situation that would enable us to evaluate the hepatoprotective effects of LF against hepatic injury. Our results showed that DMN-induced hepatic pathological damage significantly decreased the body weight and liver index, increased the mRNA and protein levels of collagen α-1(I) (ColIα-1) and α-smooth muscle actin, and increased the hydroxyproline content. However, treatment with LF significantly increased body weight and liver index, decreased the mRNA and protein levels of ColIα-1 and α-smooth muscle actin, and suppressed the hydroxyproline content when compared with the DMN-treated group. Liver histopathology also showed that low-dose LF (100. mg/kg of body weight) or high-dose LF (300. mg/kg of body weight) could significantly reduce the incidences of liver lesions induced by DMN. These results suggest that the LF exhibits potent hepatoprotection against DMN-induced liver damage in rats and that the hepatoprotective effects of LF may be due to the inhibition of collagen production and to stellate cell activation.

Original languageEnglish
Pages (from-to)3281-3291
Number of pages11
JournalJournal of Dairy Science
Volume97
Issue number6
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

liver cirrhosis
Lactoferrin
lactoferrin
Liver Cirrhosis
Dimethylnitrosamine
liver
Liver
rats
Body Weight
hepatoprotective effect
cells
body weight
hydroxyproline
Hydroxyproline
smooth muscle
actin
Smooth Muscle
collagen
Actins
Collagen

Keywords

  • Dimethylnitrosamine
  • Hepatic stellate cells
  • Lactoferrin
  • Liver fibrosis

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Lactoferrin protects against chemical-induced rat liver fibrosis by inhibiting stellate cell activation. / Tung, Yu Tang; Tang, Ting Yu; Chen, Hsiao Ling; Yang, Shang Hsun; Chong, Kowit Yu; Cheng, Winston T K; Chen, Chuan Mu.

In: Journal of Dairy Science, Vol. 97, No. 6, 2014, p. 3281-3291.

Research output: Contribution to journalArticle

Tung, Yu Tang ; Tang, Ting Yu ; Chen, Hsiao Ling ; Yang, Shang Hsun ; Chong, Kowit Yu ; Cheng, Winston T K ; Chen, Chuan Mu. / Lactoferrin protects against chemical-induced rat liver fibrosis by inhibiting stellate cell activation. In: Journal of Dairy Science. 2014 ; Vol. 97, No. 6. pp. 3281-3291.
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