Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cells in vitro

Tzou Yien Lin, Chishih Chu, Cheng Hsun Chiu

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Enterovirus 71 (EV71), the newest member of Enterovirudae, is notable for its etiological role in epidemics of severe neurological diseases in children. It appears to be emerging as an important virulent neurotropic enterovirus in the upcoming era of poliomyelitis eradication, whereas no effective vaccine or antiviral agents are available at this moment. Human and bovine lactoferrins, iron-binding proteins belonging to the nonimmune defense system, were assayed in vitro to assess their inhibiting capacity on the cytopathic effect of EV71 on human embryonal rhabdomyosarcoma (RD) cells. Both bovine and human lactoferrins were found to be potent inhibitors of EV71 infection (mean IC50, 10.5-24.5 μg/mL and 103.3-185.0 μg/ mL, respectively). Lactoferrin probably exerts its effect at the level of viral adsorption, since the ongoing infection could not be further inhibited after the EV71 penetrated RD cells.

Original languageEnglish
Pages (from-to)1161-1164
Number of pages4
JournalJournal of Infectious Diseases
Volume186
Issue number8
DOIs
Publication statusPublished - Oct 15 2002
Externally publishedYes

Fingerprint

Enterovirus Infections
Embryonal Rhabdomyosarcoma
Lactoferrin
Enterovirus
Human Enterovirus A
Iron-Binding Proteins
Rhabdomyosarcoma
Poliomyelitis
Inhibitory Concentration 50
Adsorption
Antiviral Agents
Vaccines
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cells in vitro. / Lin, Tzou Yien; Chu, Chishih; Chiu, Cheng Hsun.

In: Journal of Infectious Diseases, Vol. 186, No. 8, 15.10.2002, p. 1161-1164.

Research output: Contribution to journalArticle

@article{94b991bcd50141748611652a2eb5304e,
title = "Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cells in vitro",
abstract = "Enterovirus 71 (EV71), the newest member of Enterovirudae, is notable for its etiological role in epidemics of severe neurological diseases in children. It appears to be emerging as an important virulent neurotropic enterovirus in the upcoming era of poliomyelitis eradication, whereas no effective vaccine or antiviral agents are available at this moment. Human and bovine lactoferrins, iron-binding proteins belonging to the nonimmune defense system, were assayed in vitro to assess their inhibiting capacity on the cytopathic effect of EV71 on human embryonal rhabdomyosarcoma (RD) cells. Both bovine and human lactoferrins were found to be potent inhibitors of EV71 infection (mean IC50, 10.5-24.5 μg/mL and 103.3-185.0 μg/ mL, respectively). Lactoferrin probably exerts its effect at the level of viral adsorption, since the ongoing infection could not be further inhibited after the EV71 penetrated RD cells.",
author = "Lin, {Tzou Yien} and Chishih Chu and Chiu, {Cheng Hsun}",
year = "2002",
month = "10",
day = "15",
doi = "10.1086/343809",
language = "English",
volume = "186",
pages = "1161--1164",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cells in vitro

AU - Lin, Tzou Yien

AU - Chu, Chishih

AU - Chiu, Cheng Hsun

PY - 2002/10/15

Y1 - 2002/10/15

N2 - Enterovirus 71 (EV71), the newest member of Enterovirudae, is notable for its etiological role in epidemics of severe neurological diseases in children. It appears to be emerging as an important virulent neurotropic enterovirus in the upcoming era of poliomyelitis eradication, whereas no effective vaccine or antiviral agents are available at this moment. Human and bovine lactoferrins, iron-binding proteins belonging to the nonimmune defense system, were assayed in vitro to assess their inhibiting capacity on the cytopathic effect of EV71 on human embryonal rhabdomyosarcoma (RD) cells. Both bovine and human lactoferrins were found to be potent inhibitors of EV71 infection (mean IC50, 10.5-24.5 μg/mL and 103.3-185.0 μg/ mL, respectively). Lactoferrin probably exerts its effect at the level of viral adsorption, since the ongoing infection could not be further inhibited after the EV71 penetrated RD cells.

AB - Enterovirus 71 (EV71), the newest member of Enterovirudae, is notable for its etiological role in epidemics of severe neurological diseases in children. It appears to be emerging as an important virulent neurotropic enterovirus in the upcoming era of poliomyelitis eradication, whereas no effective vaccine or antiviral agents are available at this moment. Human and bovine lactoferrins, iron-binding proteins belonging to the nonimmune defense system, were assayed in vitro to assess their inhibiting capacity on the cytopathic effect of EV71 on human embryonal rhabdomyosarcoma (RD) cells. Both bovine and human lactoferrins were found to be potent inhibitors of EV71 infection (mean IC50, 10.5-24.5 μg/mL and 103.3-185.0 μg/ mL, respectively). Lactoferrin probably exerts its effect at the level of viral adsorption, since the ongoing infection could not be further inhibited after the EV71 penetrated RD cells.

UR - http://www.scopus.com/inward/record.url?scp=0037108680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037108680&partnerID=8YFLogxK

U2 - 10.1086/343809

DO - 10.1086/343809

M3 - Article

C2 - 12355368

AN - SCOPUS:0037108680

VL - 186

SP - 1161

EP - 1164

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 8

ER -