Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

Wen Lo, Tsung Jen Wang, Wei Liang Chen, Chiu Mei Hsueh, Shean Jen Chen, Yang Fang Chen, Hsiu Chu Chou, Pi Jung Lin, Fung Rong Hu, Chen Yuan Dong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

Original languageEnglish
Article number036005
JournalJournal of Biomedical Optics
Volume15
Issue number3
DOIs
Publication statusPublished - May 2010

Keywords

  • Collagen
  • Keratocyte activation
  • Multiphoton autofluorescence excitation
  • Photorefractive keratectomy
  • Secondharmonic generation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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