Label-free DNA detection using two-dimensional periodic relief grating as a visualized platform for diagnosis of breast cancer recurrence after surgery

Jem Kun Chen, Gang Yan Zhou, Chi Jung Chang, Ai Wei Lee, Feng Chih Chang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study we fabricated a nanopillar array of silicon oxide, involving very-large-scale integration (VLSI) and reactive ion etching (RIE), as two-dimensional periodic relief gratings (2DPRGs) on Si surfaces. Thiolated oligonucleotide was successively immobilized on the thiol functionalized surfaces of 2DPRGs by disulfide bond as an optical probe to detect a human genomic DNA (hgDNA584), related to breast cancer recurrence after surgery, from a biological specimen. The oligonucleotide-bound 2DPRG alone produces insignificant structure change, but upon hybridization with hgDNA584 leads to a dramatic change of the pillar scale due to hgDNA584 filling inside the 2DPRG layers. The performance of the sensor was evaluated by capturing hgDNA584 on the oligonucleotide-bound 2DPRGs and measuring the effective refractive index (neff), resulting of color change from pure blue to red, observed by naked eyes along an incident angle of 20-30°. The surface-bound 2DPRG based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protocol, displaying attributes of both detection methodologies: the high sensitivity and selectivity afforded by 2DPRG probes and the experimental simplicity, and miniaturization potential provided by the diffraction-based sensing technology.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalBiosensors and Bioelectronics
Volume54
DOIs
Publication statusPublished - Apr 15 2014
Externally publishedYes

Fingerprint

Oligonucleotides
Surgery
Labels
DNA
Breast Neoplasms
Recurrence
Miniaturization
Signal transduction
Refractometry
VLSI circuits
Silicon oxides
Reactive ion etching
Diffraction gratings
Silicon
Sulfhydryl Compounds
Disulfides
Oxides
Assays
Signal Transduction
Refractive index

Keywords

  • DNA detection
  • Effective medium theory
  • Refractive index
  • Two-dimensional periodic grating

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry
  • Medicine(all)

Cite this

Label-free DNA detection using two-dimensional periodic relief grating as a visualized platform for diagnosis of breast cancer recurrence after surgery. / Chen, Jem Kun; Zhou, Gang Yan; Chang, Chi Jung; Lee, Ai Wei; Chang, Feng Chih.

In: Biosensors and Bioelectronics, Vol. 54, 15.04.2014, p. 35-41.

Research output: Contribution to journalArticle

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