Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor

Chi Chang Wu, Tung Ming Pan, Chung Shu Wu, Li Chen Yen, Cheng Keng Chuang, See Tong Pang, Yuh Shyong Yang, Fu Hsiang Ko

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this study, we proposed a silicon nanobelt field-effect transistor (FET) to detect prostate specific antigen (PSA). The nanobelt FET device displayed n-channel depletion characteristics. The immobilization of prostate specific antibody (anti-PSA) molecules was attached onto the nanobelt FET surface by using the aldehyde groups of glutaraldehyde linked to the amino groups of 3-aminopropyltriethoxysilane (APTES). The shift in the drain current vs time curves of a nanobelt FET biosensor revealed that the electrical signal had a logarithmic relationship with respect to the concentration of the PSA, and detection capability was estimated in the 5 pg/mL level. To enhance the sensitivity of a nanobelt FET biosensor, this biosensor was designed by inserting arginine molecules between glutaraldehyde and APTES. Therefore, the detection capability of the developed sensor was extended to 50 fg/mL. Also, the relationship between the current shift and the logarithm of PSA concentration was exhibited linearity in the range 50 fg/mL - 500 pg/mL. The excellent electrical results of this label-free PSA nanobelt FET biosensor suggested that such biosensor might be potentially useful tools for biological research and future prostate cancer screening.

Original languageEnglish
Pages (from-to)4432-4442
Number of pages11
JournalInternational Journal of Electrochemical Science
Volume7
Issue number5
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Nanobelts
Silicon
Prostate-Specific Antigen
Antigens
Field effect transistors
Labels
Biosensors
Glutaral
Arginine
Molecules
Drain current
Aldehydes
Antibodies
Anti-Idiotypic Antibodies
Screening
Sensors

Keywords

  • Biosensor
  • Field-effect transistor
  • Label-free detection
  • Nanobelt
  • Prostate specific antigen

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Wu, C. C., Pan, T. M., Wu, C. S., Yen, L. C., Chuang, C. K., Pang, S. T., ... Ko, F. H. (2012). Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor. International Journal of Electrochemical Science, 7(5), 4432-4442.

Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor. / Wu, Chi Chang; Pan, Tung Ming; Wu, Chung Shu; Yen, Li Chen; Chuang, Cheng Keng; Pang, See Tong; Yang, Yuh Shyong; Ko, Fu Hsiang.

In: International Journal of Electrochemical Science, Vol. 7, No. 5, 2012, p. 4432-4442.

Research output: Contribution to journalArticle

Wu, CC, Pan, TM, Wu, CS, Yen, LC, Chuang, CK, Pang, ST, Yang, YS & Ko, FH 2012, 'Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor', International Journal of Electrochemical Science, vol. 7, no. 5, pp. 4432-4442.
Wu, Chi Chang ; Pan, Tung Ming ; Wu, Chung Shu ; Yen, Li Chen ; Chuang, Cheng Keng ; Pang, See Tong ; Yang, Yuh Shyong ; Ko, Fu Hsiang. / Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor. In: International Journal of Electrochemical Science. 2012 ; Vol. 7, No. 5. pp. 4432-4442.
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