l-glutamine regulates the expression of matrix proteins, pro-inflammatory cytokines and catabolic enzymes in interleukin-1beta-stimulated human chondrocytes

Kai Chiang Yang, Hsien Te Chen, Chang Chin Wu, Ya Jyun Lian, Li Lun Chen, Shoichiro Sumi, Teng Le Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Osteoarthritis (OA) of the knee is a major cause of mobility impairment. A number of studies demonstrated the therapeutic effect of l-glutamine (Gln) on different cells and tissues. However, little research has addressed the potential use of Gln on the treatment of OA. In this study, the regulation of Gln on OA-related mRNA expression and cytokine production in chondrocytes was evaluated. Human chondrocytes were stimulated with interleukin-1β (IL-1β) and subsequently cultured in Gln-supplemented medium. The results showed that Gln prevented the hypertrophic transformation and promoted the proliferation rate of IL-1β-stimulated chondrocytes. Gln also downregulated the mRNA expressions of type I collagen, IL-1β, and tumor necrosis factor-alpha (TNF-α) in stimulated cells. In addition, Gln restored the aggrecan and upregulated the TIMP-1 expressions. However, the matrix metalloproteinase-3 (MMP-3) and MMP-9 levels were highly expressed in IL-1β-stimulated chondrocytes and Gln treatment did not show any effects. Although Gln decreased the protein productions of IL-1β and TNF-α, the MMP-3 still highly produced. However, the production of tissue inhibitor of metalloproteinase-1 significantly increased. Our results suggest that Gln possesses anti-inflammatory and structure-protective properties in terms of preventing the hypertrophic transformation, regulating the productions of matrix proteins and catabolic enzymes in human chondrocytes.

Original languageEnglish
Pages (from-to)414-421
Number of pages8
JournalProcess Biochemistry
Volume51
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Chondrocytes
Glutamine
Interleukin-1beta
Enzymes
Cytokines
Proteins
Interleukin-1
Tissue
Matrix Metalloproteinase 3
Collagen
Tissue Inhibitor of Metalloproteinase-1
Osteoarthritis
Tumor Necrosis Factor-alpha
Aggrecans
Messenger RNA
Knee Osteoarthritis
Metalloproteases
Therapeutic Uses
Collagen Type I
Matrix Metalloproteinases

Keywords

  • Chondrocyte
  • l-glutamine
  • Osteoarthritis
  • Pro-inflammatory cytokine

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

l-glutamine regulates the expression of matrix proteins, pro-inflammatory cytokines and catabolic enzymes in interleukin-1beta-stimulated human chondrocytes. / Yang, Kai Chiang; Chen, Hsien Te; Wu, Chang Chin; Lian, Ya Jyun; Chen, Li Lun; Sumi, Shoichiro; Huang, Teng Le.

In: Process Biochemistry, Vol. 51, No. 3, 01.03.2016, p. 414-421.

Research output: Contribution to journalArticle

Yang, Kai Chiang ; Chen, Hsien Te ; Wu, Chang Chin ; Lian, Ya Jyun ; Chen, Li Lun ; Sumi, Shoichiro ; Huang, Teng Le. / l-glutamine regulates the expression of matrix proteins, pro-inflammatory cytokines and catabolic enzymes in interleukin-1beta-stimulated human chondrocytes. In: Process Biochemistry. 2016 ; Vol. 51, No. 3. pp. 414-421.
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