Abstract

Background. Survivin is a potential therapeutic target for cancer. Increased survivin expression promotes cell survival and therapeutic resistance. However, there is little information regarding whether the expression level of survivin affects curcumin treatment in hepatocellular carcinoma (HCC). Methods. Survivin expression was suppressed in HCC cells using a short interfering RNA (siRNA) technique. The anticancer effects of curcumin were examined using a biosensor system, MTT assay, TUNEL assay, and cell cycle analysis. Results. Curcumin resistance developed in cells with suppressed survivin, in contrast to the parental cells, as determined by survival assays. Cell cycle analysis and TUNEL assays revealed that the apoptotic cell population was increased in the scrambled-siRNA cells treated with curcumin compared with the survivin-siRNA cells. Suppression of survivin expression resulted in curcumin resistance via the modulation of Bcl-2 and Bax expression. Conclusions. We conclude that the expression levels of survivin may mediate the therapeutic efficacy of curcumin in HCC cells.

Original languageEnglish
Pages (from-to)3547-3555
Number of pages9
JournalAnnals of Surgical Oncology
Volume19
Issue number11
DOIs
Publication statusPublished - Oct 2012

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Curcumin
Hepatocellular Carcinoma
Small Interfering RNA
In Situ Nick-End Labeling
Cell Cycle
Biosensing Techniques
Cell Survival
Therapeutics
Population

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

@article{375742a569244f3d8ca0e7d6ff6fd82f,
title = "Knockdown survivin expression reduces the efficacy of curcumin treatment in hepatocellular carcinoma cells",
abstract = "Background. Survivin is a potential therapeutic target for cancer. Increased survivin expression promotes cell survival and therapeutic resistance. However, there is little information regarding whether the expression level of survivin affects curcumin treatment in hepatocellular carcinoma (HCC). Methods. Survivin expression was suppressed in HCC cells using a short interfering RNA (siRNA) technique. The anticancer effects of curcumin were examined using a biosensor system, MTT assay, TUNEL assay, and cell cycle analysis. Results. Curcumin resistance developed in cells with suppressed survivin, in contrast to the parental cells, as determined by survival assays. Cell cycle analysis and TUNEL assays revealed that the apoptotic cell population was increased in the scrambled-siRNA cells treated with curcumin compared with the survivin-siRNA cells. Suppression of survivin expression resulted in curcumin resistance via the modulation of Bcl-2 and Bax expression. Conclusions. We conclude that the expression levels of survivin may mediate the therapeutic efficacy of curcumin in HCC cells.",
author = "Hung, {Chin Sheng} and Liu, {Hui Hsiung} and Huang, {Ming Te} and Cheng, {Chao Wen} and Kuo, {Li Jen} and Ho, {Yuan Soon} and Wu, {Chih Hsiung} and Chen, {Chien Ming} and Wei, {Po Li} and Chang, {Yu Jia}",
year = "2012",
month = "10",
doi = "10.1245/s10434-012-2393-4",
language = "English",
volume = "19",
pages = "3547--3555",
journal = "Annals of Surgical Oncology",
issn = "1068-9265",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - Knockdown survivin expression reduces the efficacy of curcumin treatment in hepatocellular carcinoma cells

AU - Hung, Chin Sheng

AU - Liu, Hui Hsiung

AU - Huang, Ming Te

AU - Cheng, Chao Wen

AU - Kuo, Li Jen

AU - Ho, Yuan Soon

AU - Wu, Chih Hsiung

AU - Chen, Chien Ming

AU - Wei, Po Li

AU - Chang, Yu Jia

PY - 2012/10

Y1 - 2012/10

N2 - Background. Survivin is a potential therapeutic target for cancer. Increased survivin expression promotes cell survival and therapeutic resistance. However, there is little information regarding whether the expression level of survivin affects curcumin treatment in hepatocellular carcinoma (HCC). Methods. Survivin expression was suppressed in HCC cells using a short interfering RNA (siRNA) technique. The anticancer effects of curcumin were examined using a biosensor system, MTT assay, TUNEL assay, and cell cycle analysis. Results. Curcumin resistance developed in cells with suppressed survivin, in contrast to the parental cells, as determined by survival assays. Cell cycle analysis and TUNEL assays revealed that the apoptotic cell population was increased in the scrambled-siRNA cells treated with curcumin compared with the survivin-siRNA cells. Suppression of survivin expression resulted in curcumin resistance via the modulation of Bcl-2 and Bax expression. Conclusions. We conclude that the expression levels of survivin may mediate the therapeutic efficacy of curcumin in HCC cells.

AB - Background. Survivin is a potential therapeutic target for cancer. Increased survivin expression promotes cell survival and therapeutic resistance. However, there is little information regarding whether the expression level of survivin affects curcumin treatment in hepatocellular carcinoma (HCC). Methods. Survivin expression was suppressed in HCC cells using a short interfering RNA (siRNA) technique. The anticancer effects of curcumin were examined using a biosensor system, MTT assay, TUNEL assay, and cell cycle analysis. Results. Curcumin resistance developed in cells with suppressed survivin, in contrast to the parental cells, as determined by survival assays. Cell cycle analysis and TUNEL assays revealed that the apoptotic cell population was increased in the scrambled-siRNA cells treated with curcumin compared with the survivin-siRNA cells. Suppression of survivin expression resulted in curcumin resistance via the modulation of Bcl-2 and Bax expression. Conclusions. We conclude that the expression levels of survivin may mediate the therapeutic efficacy of curcumin in HCC cells.

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DO - 10.1245/s10434-012-2393-4

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VL - 19

SP - 3547

EP - 3555

JO - Annals of Surgical Oncology

JF - Annals of Surgical Oncology

SN - 1068-9265

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