Knockdown of POLDIP2 suppresses tumor growth and invasion capacity and is linked to unfavorable transformation ability and metastatic feature in non-small cell lung cancer

Ying Chieh Chen, Chih Chi Kuo, Chih Feng Chian, Ching Tzao, Shan Yueh Chang, Yu Lueng Shih, Ya Wen Lin, Mu Hsien Yu, Her Young Su

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Abstract

The main problem in the treatment of non-small cell lung cancer (NSCLC) is metastasis. Epithelial–mesenchymal transition (EMT) is known as the critical signaling in tumor progression, metastasis, and also the drug resistance. In this study, we reported a novel gene Polymerase delta-interacting protein 2 (POLDIP2) was downregulated in NSCLC tissues and first demonstrated that overexpression of POLDIP2 increased the anchorage-independent growth (AIG) and invasiveness of H1299 cells. In addition, we examined that knockdown of POLDIP2 in H1299 and A549 cells reduced tumorigenicity and metastatic capacity in vitro and also in vivo. Moreover, downregulation of the cell proliferation marker cyclin D1 and EMT markers CDH2, Slug, and Twist was showed in H1299 cells by POLDIP2 knockdown, suggesting that the inhibition of malignancy was affected by modulating key genes for tumor growth and invasiveness. Taken together, our study is the first study that demonstrated that POLDIP2 gene was function as an oncogene in NSCLC and implied the oncogenic ability might be through promoting cell proliferation or EMT.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalExperimental Cell Research
Volume368
Issue number1
DOIs
Publication statusPublished - Jul 1 2018

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Non-Small Cell Lung Carcinoma
Growth
Neoplasms
Down-Regulation
Cell Proliferation
Neoplasm Metastasis
Genes
Gastropoda
Cyclin D1
Oncogenes
Drug Resistance
delta protein

Keywords

  • Invasion capacity
  • Metastatic feature
  • Non-small cell lung cancer
  • POLDIP2
  • Tumor growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

Knockdown of POLDIP2 suppresses tumor growth and invasion capacity and is linked to unfavorable transformation ability and metastatic feature in non-small cell lung cancer. / Chen, Ying Chieh; Kuo, Chih Chi; Chian, Chih Feng; Tzao, Ching; Chang, Shan Yueh; Shih, Yu Lueng; Lin, Ya Wen; Yu, Mu Hsien; Su, Her Young.

In: Experimental Cell Research, Vol. 368, No. 1, 01.07.2018, p. 42-49.

Research output: Contribution to journalArticle

Chen, Ying Chieh ; Kuo, Chih Chi ; Chian, Chih Feng ; Tzao, Ching ; Chang, Shan Yueh ; Shih, Yu Lueng ; Lin, Ya Wen ; Yu, Mu Hsien ; Su, Her Young. / Knockdown of POLDIP2 suppresses tumor growth and invasion capacity and is linked to unfavorable transformation ability and metastatic feature in non-small cell lung cancer. In: Experimental Cell Research. 2018 ; Vol. 368, No. 1. pp. 42-49.
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