Kinetic analysis on the sensitivity of glucose- or glyoxal-induced LDL glycation to the inhibitory effect of Psidium guajava extract in a physiomimic system

Chiu Lan Hsieh, Ming Hua Yang, Charng Cherng Chyau, Chun Hon Chiu, Hui Er Wang, Yuh Charn Lin, Wen Ta Chiu, Robert Y. Peng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Experimentation with a physiomimic system and kinetic analysis exhibited four distinct reaction phases in LDL glycation despite of the type of inducer: glucose or glyoxal. LDL glycation was more sensitive to a status of hyperglycemia (such as 400 mg glucose/100 mL) as evidenced by the reaction order of 0.53. Glucose reacted intensively in the Initial Phase (reaction period 0-2 h) which was identified to result from a parallel mechanism involving both the direct Schiff's product formation and the auto-oxidative cleavages. In contrast, a physiological level of glyoxal revealed merely a reaction order of only 0.09, implicitly indicating a far less sensitive glycation which can be attributed to a mechanism proceeding simply through a molecular Schiff's reaction. On treatment with Psidium guajava L. aqueous extract (PE) (0.01-0.625 mg/mL), a rather unique and significant inhibitory characteristic on LDL glycation was observed with a dose-dependent manner. We attributed such an effect of PE to its distinct abundance of polyphenolic content (165.61 ± 10.39 mg gallic acid equivalent (GAE)/g). Conclusively, PE is an excellent anti-LDL glycative agent whose potential therapeutic uses can be extended to the prevention of a variety of cardiovascular and neurodegenerative diseases associated with glycations.

Original languageEnglish
Pages (from-to)92-100
Number of pages9
JournalBioSystems
Volume88
Issue number1-2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Glyoxal
Psidium
glycation
Psidium guajava
Glucose
glucose
Kinetics
kinetics
extracts
Neurodegenerative diseases
Gallic Acid
Therapeutic Uses
Hyperglycemia
Neurodegenerative Diseases
cleavage
Distinct
Cardiovascular Diseases
Parallel Mechanism
neurodegenerative diseases
hyperglycemia

Keywords

  • Glucose
  • Glyoxal
  • Kinetic analysis
  • LDL glycation
  • Psidium guajava L.

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Drug Discovery

Cite this

Kinetic analysis on the sensitivity of glucose- or glyoxal-induced LDL glycation to the inhibitory effect of Psidium guajava extract in a physiomimic system. / Hsieh, Chiu Lan; Yang, Ming Hua; Chyau, Charng Cherng; Chiu, Chun Hon; Wang, Hui Er; Lin, Yuh Charn; Chiu, Wen Ta; Peng, Robert Y.

In: BioSystems, Vol. 88, No. 1-2, 03.2007, p. 92-100.

Research output: Contribution to journalArticle

Hsieh, Chiu Lan ; Yang, Ming Hua ; Chyau, Charng Cherng ; Chiu, Chun Hon ; Wang, Hui Er ; Lin, Yuh Charn ; Chiu, Wen Ta ; Peng, Robert Y. / Kinetic analysis on the sensitivity of glucose- or glyoxal-induced LDL glycation to the inhibitory effect of Psidium guajava extract in a physiomimic system. In: BioSystems. 2007 ; Vol. 88, No. 1-2. pp. 92-100.
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