Kefir supplementation modifies gut microbiota composition, reduces physical fatigue, and improves exercise performance in mice

Yi Ju Hsu, Wen Ching Huang, Jin Seng Lin, Yi Ming Chen, Shang Tse Ho, Chi Chang Huang, Yu Tang Tung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. The present study evaluated the potential beneficial effect of kefir (KF) against fatigue. Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition. Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively. The gut microbiota was analyzed using 16S rRNA gene sequencing. The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups. The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group. In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise. The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group. KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test. However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation. Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.
Original languageEnglish
JournalNutrients
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 4 2018

Fingerprint

kefir
intestinal microorganisms
Fatigue
exercise
mice
Gastrointestinal Microbiome
Kefir
Forelimb
Blood Urea Nitrogen
Hand Strength
creatine kinase
Creatine Kinase
forelimbs
urea nitrogen
blood serum
Ammonia
lactates
Lactic Acid
ammonia
Bacteroidetes

Keywords

  • Antifatigue
  • Exercise performance
  • Gut microbiota
  • Kefir

Cite this

Kefir supplementation modifies gut microbiota composition, reduces physical fatigue, and improves exercise performance in mice. / Hsu, Yi Ju; Huang, Wen Ching; Lin, Jin Seng; Chen, Yi Ming; Ho, Shang Tse; Huang, Chi Chang; Tung, Yu Tang.

In: Nutrients, Vol. 10, No. 7, 04.07.2018.

Research output: Contribution to journalArticle

Hsu, Yi Ju ; Huang, Wen Ching ; Lin, Jin Seng ; Chen, Yi Ming ; Ho, Shang Tse ; Huang, Chi Chang ; Tung, Yu Tang. / Kefir supplementation modifies gut microbiota composition, reduces physical fatigue, and improves exercise performance in mice. In: Nutrients. 2018 ; Vol. 10, No. 7.
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AB - © 2018 by the authors. Licensee MDPI, Basel, Switzerland. The present study evaluated the potential beneficial effect of kefir (KF) against fatigue. Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition. Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively. The gut microbiota was analyzed using 16S rRNA gene sequencing. The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups. The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group. In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise. The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group. KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test. However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation. Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue.

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