Kaposi's sarcoma-associated herpesvirus encodes a bZIP protein with homology to BZLF1 of Epstein-Barr virus

Su Fang Lin, Dan R. Robinson, George Miller, Hsing Jien Kung

Research output: Contribution to journalArticle

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Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is a recently discovered human gamma herpesvirus strongly implicated in AIDS-related neoplasms. We report here the identification in the KSHV genome of a gene for a protein designated K-bZIP and belonging to the basic-leucine zipper (bZIP) family of transcription factors. K-bZIP shows significant homology to BZLF1, which plays a key role in the replication and reactivation of Epstein-Barr virus. K-bZIP is a homodimerizing protein of 237 amino acids with a prototypic bZIP domain at the C terminus. The N-terminal portion of K-bZIP is derived from the K8 open reading frame which, through in-frame splicing, adjoins the ZIP domain. This structure was revealed by rapid analysis of cDNA ends, followed by cloning of the entire cDNA. A 1.35-kb transcript encoding K-bZIP was detected in BCBL-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate. The synthesis of this transcript was blocked by the protein synthesis inhibitor cycloheximide but not by the viral DNA synthesis inhibitor phosphonoacetate, a result which classifies it as an early lytic gene. RNase protection analysis further mapped the major transcription start site for the 1.35-kb K, bZIP mRNA and identified two other splice variants which encode proteins with the N-terminal portion of K-bZIP but lacking the C-terminal ZIP domain. Full-length K-bZIP forms dimers with itself, and the C terminus encompassing the ZIP domain is required for this process. Our studies set the stage for understanding the role of K-bZIP in the replication and reactivatlon of the KSHV genome.

Original languageEnglish
Pages (from-to)1909-1917
Number of pages9
JournalJournal of Virology
Volume73
Issue number3
Publication statusPublished - Mar 4 1999
Externally publishedYes

Fingerprint

Human herpesvirus 8
Leucine Zippers
Human herpesvirus 4
Human Herpesvirus 8
leucine zipper
Human Herpesvirus 4
Proteins
proteins
Complementary DNA
Phosphonoacetic Acid
Basic-Leucine Zipper Transcription Factors
Genome
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
protein synthesis inhibitors
Herpesviridae
Transcription Initiation Site
Viral DNA
synthesis
genome

ASJC Scopus subject areas

  • Immunology

Cite this

Kaposi's sarcoma-associated herpesvirus encodes a bZIP protein with homology to BZLF1 of Epstein-Barr virus. / Lin, Su Fang; Robinson, Dan R.; Miller, George; Kung, Hsing Jien.

In: Journal of Virology, Vol. 73, No. 3, 04.03.1999, p. 1909-1917.

Research output: Contribution to journalArticle

Lin, Su Fang ; Robinson, Dan R. ; Miller, George ; Kung, Hsing Jien. / Kaposi's sarcoma-associated herpesvirus encodes a bZIP protein with homology to BZLF1 of Epstein-Barr virus. In: Journal of Virology. 1999 ; Vol. 73, No. 3. pp. 1909-1917.
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