Kainic acid-induced neurotrophic activities in developing cortical neurons

Yi Hsuan Lee, Kwang Ming Fang, Chuen Mao Yang, Hwa Min Hwang, Chi Tso Chiu, Wuhong Tsai

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Using primary cultured cortical neurons from embryonic rat brains, we elucidated an α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainic acid (KA) receptor-mediated neuroprotective mechanism through actions of nerve growth factor (NGF) in developing neurons. Neurotoxicity of KA in early days in vitro neurons was quite low compared with the mature neurons. However, pretreatment with anti-NGF antibody or TrkA inhibitor AG- 879 profoundly raised KA toxicity. Furthermore, KA stimulation resulted in an increase of Trk A expression and phosphorylation, which was blocked not only by the AMPA/KA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione and AG-879, but also by the phospholipase C inhibitor U73122 and the intracellular calcium chelator BAPTA. A study of polyphosphoinositide turnover showed that KA-stimulated phospholipase C (PLC) activity was directly triggered by the AMPA/KA receptor activity, but not by the activity of TrkA or other excitatory amino acid receptor subtypes. Sources of KA- increased intracellular calcium levels were contributed by both extracellular calcium influx and intracellular calcium release and were partially sensitive to guanosine 5'-0-(2-thiodiphosphate). These results indicate that in developing cortical neurons, activation of AMPA/KA receptors by KA may induce expression, followed by activation of TrkA via PLC signaling and intracellular calcium elevation and hence increase reception of NGF on KA- challenged neurons. A G protein-coupled AMPA/KA receptor may be involved in these metabotropic events for neuronal protection.

Original languageEnglish
Pages (from-to)2401-2411
Number of pages11
JournalJournal of Neurochemistry
Volume74
Issue number6
DOIs
Publication statusPublished - 2000

Fingerprint

Kainic Acid
Kainic Acid Receptors
Neurons
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Type C Phospholipases
Nerve Growth Factor
Calcium
Chemical activation
6-Cyano-7-nitroquinoxaline-2,3-dione
Isoxazoles
Phosphatidylinositol Phosphates
Phosphorylation
Calcium Signaling
Guanosine
Propionates
Glutamate Receptors
GTP-Binding Proteins
Toxicity
Rats
Brain

Keywords

  • α-Amino-3-hydroxy-5-methyl-4-isoxazole propionate/kainic acid receptors
  • Calcium
  • Kainic acid
  • Nerve growth factor
  • Neuroprotection
  • Phospholipase C

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Lee, Y. H., Fang, K. M., Yang, C. M., Hwang, H. M., Chiu, C. T., & Tsai, W. (2000). Kainic acid-induced neurotrophic activities in developing cortical neurons. Journal of Neurochemistry, 74(6), 2401-2411. https://doi.org/10.1046/j.1471-4159.2000.0742401.x

Kainic acid-induced neurotrophic activities in developing cortical neurons. / Lee, Yi Hsuan; Fang, Kwang Ming; Yang, Chuen Mao; Hwang, Hwa Min; Chiu, Chi Tso; Tsai, Wuhong.

In: Journal of Neurochemistry, Vol. 74, No. 6, 2000, p. 2401-2411.

Research output: Contribution to journalArticle

Lee, YH, Fang, KM, Yang, CM, Hwang, HM, Chiu, CT & Tsai, W 2000, 'Kainic acid-induced neurotrophic activities in developing cortical neurons', Journal of Neurochemistry, vol. 74, no. 6, pp. 2401-2411. https://doi.org/10.1046/j.1471-4159.2000.0742401.x
Lee, Yi Hsuan ; Fang, Kwang Ming ; Yang, Chuen Mao ; Hwang, Hwa Min ; Chiu, Chi Tso ; Tsai, Wuhong. / Kainic acid-induced neurotrophic activities in developing cortical neurons. In: Journal of Neurochemistry. 2000 ; Vol. 74, No. 6. pp. 2401-2411.
@article{3be6e619c44041d09c0c9f4a95605b1e,
title = "Kainic acid-induced neurotrophic activities in developing cortical neurons",
abstract = "Using primary cultured cortical neurons from embryonic rat brains, we elucidated an α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainic acid (KA) receptor-mediated neuroprotective mechanism through actions of nerve growth factor (NGF) in developing neurons. Neurotoxicity of KA in early days in vitro neurons was quite low compared with the mature neurons. However, pretreatment with anti-NGF antibody or TrkA inhibitor AG- 879 profoundly raised KA toxicity. Furthermore, KA stimulation resulted in an increase of Trk A expression and phosphorylation, which was blocked not only by the AMPA/KA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione and AG-879, but also by the phospholipase C inhibitor U73122 and the intracellular calcium chelator BAPTA. A study of polyphosphoinositide turnover showed that KA-stimulated phospholipase C (PLC) activity was directly triggered by the AMPA/KA receptor activity, but not by the activity of TrkA or other excitatory amino acid receptor subtypes. Sources of KA- increased intracellular calcium levels were contributed by both extracellular calcium influx and intracellular calcium release and were partially sensitive to guanosine 5'-0-(2-thiodiphosphate). These results indicate that in developing cortical neurons, activation of AMPA/KA receptors by KA may induce expression, followed by activation of TrkA via PLC signaling and intracellular calcium elevation and hence increase reception of NGF on KA- challenged neurons. A G protein-coupled AMPA/KA receptor may be involved in these metabotropic events for neuronal protection.",
keywords = "α-Amino-3-hydroxy-5-methyl-4-isoxazole propionate/kainic acid receptors, Calcium, Kainic acid, Nerve growth factor, Neuroprotection, Phospholipase C",
author = "Lee, {Yi Hsuan} and Fang, {Kwang Ming} and Yang, {Chuen Mao} and Hwang, {Hwa Min} and Chiu, {Chi Tso} and Wuhong Tsai",
year = "2000",
doi = "10.1046/j.1471-4159.2000.0742401.x",
language = "English",
volume = "74",
pages = "2401--2411",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Kainic acid-induced neurotrophic activities in developing cortical neurons

AU - Lee, Yi Hsuan

AU - Fang, Kwang Ming

AU - Yang, Chuen Mao

AU - Hwang, Hwa Min

AU - Chiu, Chi Tso

AU - Tsai, Wuhong

PY - 2000

Y1 - 2000

N2 - Using primary cultured cortical neurons from embryonic rat brains, we elucidated an α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainic acid (KA) receptor-mediated neuroprotective mechanism through actions of nerve growth factor (NGF) in developing neurons. Neurotoxicity of KA in early days in vitro neurons was quite low compared with the mature neurons. However, pretreatment with anti-NGF antibody or TrkA inhibitor AG- 879 profoundly raised KA toxicity. Furthermore, KA stimulation resulted in an increase of Trk A expression and phosphorylation, which was blocked not only by the AMPA/KA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione and AG-879, but also by the phospholipase C inhibitor U73122 and the intracellular calcium chelator BAPTA. A study of polyphosphoinositide turnover showed that KA-stimulated phospholipase C (PLC) activity was directly triggered by the AMPA/KA receptor activity, but not by the activity of TrkA or other excitatory amino acid receptor subtypes. Sources of KA- increased intracellular calcium levels were contributed by both extracellular calcium influx and intracellular calcium release and were partially sensitive to guanosine 5'-0-(2-thiodiphosphate). These results indicate that in developing cortical neurons, activation of AMPA/KA receptors by KA may induce expression, followed by activation of TrkA via PLC signaling and intracellular calcium elevation and hence increase reception of NGF on KA- challenged neurons. A G protein-coupled AMPA/KA receptor may be involved in these metabotropic events for neuronal protection.

AB - Using primary cultured cortical neurons from embryonic rat brains, we elucidated an α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainic acid (KA) receptor-mediated neuroprotective mechanism through actions of nerve growth factor (NGF) in developing neurons. Neurotoxicity of KA in early days in vitro neurons was quite low compared with the mature neurons. However, pretreatment with anti-NGF antibody or TrkA inhibitor AG- 879 profoundly raised KA toxicity. Furthermore, KA stimulation resulted in an increase of Trk A expression and phosphorylation, which was blocked not only by the AMPA/KA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione and AG-879, but also by the phospholipase C inhibitor U73122 and the intracellular calcium chelator BAPTA. A study of polyphosphoinositide turnover showed that KA-stimulated phospholipase C (PLC) activity was directly triggered by the AMPA/KA receptor activity, but not by the activity of TrkA or other excitatory amino acid receptor subtypes. Sources of KA- increased intracellular calcium levels were contributed by both extracellular calcium influx and intracellular calcium release and were partially sensitive to guanosine 5'-0-(2-thiodiphosphate). These results indicate that in developing cortical neurons, activation of AMPA/KA receptors by KA may induce expression, followed by activation of TrkA via PLC signaling and intracellular calcium elevation and hence increase reception of NGF on KA- challenged neurons. A G protein-coupled AMPA/KA receptor may be involved in these metabotropic events for neuronal protection.

KW - α-Amino-3-hydroxy-5-methyl-4-isoxazole propionate/kainic acid receptors

KW - Calcium

KW - Kainic acid

KW - Nerve growth factor

KW - Neuroprotection

KW - Phospholipase C

UR - http://www.scopus.com/inward/record.url?scp=0034028560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034028560&partnerID=8YFLogxK

U2 - 10.1046/j.1471-4159.2000.0742401.x

DO - 10.1046/j.1471-4159.2000.0742401.x

M3 - Article

C2 - 10820201

AN - SCOPUS:0034028560

VL - 74

SP - 2401

EP - 2411

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -