Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid β-Oxidation

Yu Ting Kao, Bi Lan Chang, Jian Jong Liang, Hang Jen Tsai, Yi Ling Lee, Ren Jye Lin, Yi Ling Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Infection with Japanese encephalitis virus (JEV) can induce the expression of pro-inflammatory cytokines and cause acute encephalitis in humans. β-oxidation breaks down fatty acids for ATP production in mitochondria, and impaired β-oxidation can induce pro-inflammatory cytokine expression. To address the role of fatty-acid β-oxidation in JEV infection, we measured the oxygen consumption rate of mock- and JEV-infected cells cultured with or without long chain fatty acid (LCFA) palmitate. Cells with JEV infection showed impaired LCFA β-oxidation and increased interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) expression. JEV nonstructural protein 5 (NS5) interacted with hydroxyacyl-CoA dehydrogenase α and β subunits, two components of the mitochondrial trifunctional protein (MTP) involved in LCFA β-oxidation, and NS5 proteins were detected in mitochondria and co-localized with MTP. LCFA β-oxidation was impaired and higher cytokines were induced in cells overexpressing NS5 protein as compared with control cells. Deletion and mutation studies showed that the N-terminus of NS5 was involved in the MTP association, and a single point mutation of NS5 residue 19 from methionine to alanine (NS5-M19A) reduced its binding ability with MTP. The recombinant JEV with NS5-M19A mutation (JEV-NS5-M19A) was less able to block LCFA β-oxidation and induced lower levels of IL-6 and TNF-α than wild-type JEV. Moreover, mice challenged with JEV-NS5-M19A showed less neurovirulence and neuroinvasiveness. We identified a novel function of JEV NS5 in viral pathogenesis by impairing LCFA β-oxidation and inducing cytokine expression by association with MTP.

Original languageEnglish
Article numbere1004750
Pages (from-to)1-26
Number of pages26
JournalPLoS Pathogens
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

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Mitochondrial Trifunctional Protein
Japanese Encephalitis Virus
Fatty Acids
Proteins
Cytokines
Interleukin-6
Mitochondria
Infection
Sequence Deletion
Palmitates
Encephalitis
Coenzyme A

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid β-Oxidation. / Kao, Yu Ting; Chang, Bi Lan; Liang, Jian Jong; Tsai, Hang Jen; Lee, Yi Ling; Lin, Ren Jye; Lin, Yi Ling.

In: PLoS Pathogens, Vol. 11, No. 3, e1004750, 01.03.2015, p. 1-26.

Research output: Contribution to journalArticle

Kao, Yu Ting ; Chang, Bi Lan ; Liang, Jian Jong ; Tsai, Hang Jen ; Lee, Yi Ling ; Lin, Ren Jye ; Lin, Yi Ling. / Japanese Encephalitis Virus Nonstructural Protein NS5 Interacts with Mitochondrial Trifunctional Protein and Impairs Fatty Acid β-Oxidation. In: PLoS Pathogens. 2015 ; Vol. 11, No. 3. pp. 1-26.
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