Isothermal titration calorimetry for characterization of recombinant proteins

Lina Baranauskiene, Tai Chih Kuo, Wen Yih Chen, Daumantas Matulis

Research output: Contribution to journalReview article

Abstract

Isothermal titration calorimetry is widely used to measure the affinities and enthalpies of interaction between proteins and/or small molecules. The quantitative nature of the technique is especially useful in the characterization of recombinant proteins while determining the fraction of protein capable of binding a specific ligand and thus the protein purity. The revealed thermodynamic information sheds light on the binding mechanism, important for the targeted drug design of the biologics. Here we show examples how, together with the thermal shift assay, combination of both techniques enables characterization of protein stability and ligand binding. Furthermore, the binding-linked reactions that strongly affect the observed thermodynamic parameters and must be dissected to obtain the intrinsic parameters that are necessary for the structure-based rational drug design are being demonstrated using inhibitors of Hsp90, an anticancer target protein.

LanguageEnglish
Pages9-15
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume55
DOIs
Publication statusPublished - Feb 1 2019

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Recombinant proteins
Calorimetry
Titration
Recombinant Proteins
Drug Design
Proteins
Thermodynamics
Ligands
Protein Stability
Biological Products
Protein Binding
Hot Temperature
Pharmaceutical Preparations
Enthalpy
Assays
Molecules

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Isothermal titration calorimetry for characterization of recombinant proteins. / Baranauskiene, Lina; Kuo, Tai Chih; Chen, Wen Yih; Matulis, Daumantas.

In: Current Opinion in Biotechnology, Vol. 55, 01.02.2019, p. 9-15.

Research output: Contribution to journalReview article

Baranauskiene, Lina ; Kuo, Tai Chih ; Chen, Wen Yih ; Matulis, Daumantas. / Isothermal titration calorimetry for characterization of recombinant proteins. In: Current Opinion in Biotechnology. 2019 ; Vol. 55. pp. 9-15.
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