Isosteviol acts on potassium channels to relax isolated aortic strips of Wistar rat

Kar L. Wong, Paul Chan, Hung Y. Yang, Feng-Lin Hsu, I. M. Liu, Yu W. Cheng, Juei Tang Cheng

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Isosteviol is a derivative of stevioside, a constituent of Stevia rebaudiana, which is commonly used as a noncaloric sugar substitute in Japan and Brazil. In the present study, the role of potassium channels in the vasodilator effect of isosteviol was investigated using potassium channel blockers on isosteviol-induced relaxation of isolated aortic rings prepared from Wistar rats. Isosteviol dose-dependently relaxed the vasopressin (10 -8 M)-induced vasoconstriction in isolated aortic rings with or without endothelium. However, in the presence of potassium chloride (3×10-2 M), the vasodilator effect of isosteviol on arterial strips disappeared. Only the inhibitors specific for the ATP-sensitive potassium (KATP) channel or small conductance calcium-activated potassium (SKCa) channel inhibited the vasodilator effect of isosteviol in isolated aortic rings contracted with 10-8 M vasopressin. Also; since the isosteviol-induced relaxation was unchanged by methylene blue, a role of nitric oxide and/or endothelium in the vasodilatation produced by isosteviol could be ruled out. The obtained results indicated that vasodilatation induced by isosteviol is related to the opening of SK Ca and KATP channels.

Original languageEnglish
Pages (from-to)2379-2387
Number of pages9
JournalLife Sciences
Volume74
Issue number19
DOIs
Publication statusPublished - Mar 26 2004

Keywords

  • Aortic rings
  • Isosteviol
  • Potassium channel blockers
  • Stevia rebaudiana

ASJC Scopus subject areas

  • Pharmacology

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