Isolation of mesenchymal stem cells from human ligamentum flavum: Implicating etiology of ligamentum flavum hypertrophy

Yi Te Chen, Jyh Ding Wei, Jung Pan Wang, Hsieh Hsing Lee, En Rung Chiang, Hung Chang Lai, Ling Lan Chen, Yi Ting Lee, Chih Chien Tsai, Chien Lin Liu, Shih Chieh Hung

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Study Design.: To demonstrate the existence of mesenchymal stem cells (MSCs) in ligamentum flavum (LF) and their pathogenic role in LF hypertrophy. Objective.: To isolate and characterize LF-derived MSCs and their response to transforming growth factor-beta 1 (TGF-β1) and trichostatin A (TSA), a histone deacetylase inhibitor (HDACi). Summary of Background Data.: LF is a connective tissue, of which hypertrophic changes induce spinal stenosis. The pathogenic role of TGF-β1 in spinal stenosis has been implicated. TSA has been shown to suppress TGF-β1-induced alpha-smooth muscle actin (α-SMA), type I and III collagen synthesis in a variety of cells. MSCs have been isolated from a variety of adult tissues, except LF. Whether MSCs exist in LF and their response to TGF-β1 and TSA is not clear. Methods.: The MSCs from LF were isolated and cultured. Their phenotypic character, linage differentiation potential, and response to TGF-β1 and TSA were analyzed. Results.: LF-derived MSCs have the similar profile of surface markers as bone marrow MSCs. They were demonstrated to have the potential to be differentiated into osteoblasts, adipocytes, and chondrocytes. Administration of TGF-β1 stimulated cell proliferation, enhanced the gene expression of type I and III collagen, and increased the gene expression and protein level of α-SMA. TSA blocked the fibrogenic effects of TGF-β1. Conclusion.: The current results demonstrated the isolation of MSCs from LF. The cellular response to TGF-β1 implied that these cells might play an important role in the pathogenesis of LF hypertrophy. TSA, which blocks the effects of TGF-β1, may be a potent therapeutic choice for inhibiting LF hypertrophy.

Original languageEnglish
JournalSpine
Volume36
Issue number18
DOIs
Publication statusPublished - Aug 15 2011

Fingerprint

Ligamentum Flavum
Mesenchymal Stromal Cells
Hypertrophy
trichostatin A
Transforming Growth Factor beta
Spinal Stenosis
Collagen Type III
Collagen Type I
Gene Expression
Histone Deacetylase Inhibitors
Chondrocytes
Osteoblasts
Adipocytes
Connective Tissue
Smooth Muscle
Actins

Keywords

  • histone deacetylase inhibitor
  • ligamentum flavum
  • mesenchymal stem cells
  • spinal stenosis
  • TGF-beta1
  • trichostatin A

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Clinical Neurology

Cite this

Chen, Y. T., Wei, J. D., Wang, J. P., Lee, H. H., Chiang, E. R., Lai, H. C., ... Hung, S. C. (2011). Isolation of mesenchymal stem cells from human ligamentum flavum: Implicating etiology of ligamentum flavum hypertrophy. Spine, 36(18). https://doi.org/10.1097/BRS.0b013e3182053f58

Isolation of mesenchymal stem cells from human ligamentum flavum : Implicating etiology of ligamentum flavum hypertrophy. / Chen, Yi Te; Wei, Jyh Ding; Wang, Jung Pan; Lee, Hsieh Hsing; Chiang, En Rung; Lai, Hung Chang; Chen, Ling Lan; Lee, Yi Ting; Tsai, Chih Chien; Liu, Chien Lin; Hung, Shih Chieh.

In: Spine, Vol. 36, No. 18, 15.08.2011.

Research output: Contribution to journalArticle

Chen, YT, Wei, JD, Wang, JP, Lee, HH, Chiang, ER, Lai, HC, Chen, LL, Lee, YT, Tsai, CC, Liu, CL & Hung, SC 2011, 'Isolation of mesenchymal stem cells from human ligamentum flavum: Implicating etiology of ligamentum flavum hypertrophy', Spine, vol. 36, no. 18. https://doi.org/10.1097/BRS.0b013e3182053f58
Chen, Yi Te ; Wei, Jyh Ding ; Wang, Jung Pan ; Lee, Hsieh Hsing ; Chiang, En Rung ; Lai, Hung Chang ; Chen, Ling Lan ; Lee, Yi Ting ; Tsai, Chih Chien ; Liu, Chien Lin ; Hung, Shih Chieh. / Isolation of mesenchymal stem cells from human ligamentum flavum : Implicating etiology of ligamentum flavum hypertrophy. In: Spine. 2011 ; Vol. 36, No. 18.
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abstract = "Study Design.: To demonstrate the existence of mesenchymal stem cells (MSCs) in ligamentum flavum (LF) and their pathogenic role in LF hypertrophy. Objective.: To isolate and characterize LF-derived MSCs and their response to transforming growth factor-beta 1 (TGF-β1) and trichostatin A (TSA), a histone deacetylase inhibitor (HDACi). Summary of Background Data.: LF is a connective tissue, of which hypertrophic changes induce spinal stenosis. The pathogenic role of TGF-β1 in spinal stenosis has been implicated. TSA has been shown to suppress TGF-β1-induced alpha-smooth muscle actin (α-SMA), type I and III collagen synthesis in a variety of cells. MSCs have been isolated from a variety of adult tissues, except LF. Whether MSCs exist in LF and their response to TGF-β1 and TSA is not clear. Methods.: The MSCs from LF were isolated and cultured. Their phenotypic character, linage differentiation potential, and response to TGF-β1 and TSA were analyzed. Results.: LF-derived MSCs have the similar profile of surface markers as bone marrow MSCs. They were demonstrated to have the potential to be differentiated into osteoblasts, adipocytes, and chondrocytes. Administration of TGF-β1 stimulated cell proliferation, enhanced the gene expression of type I and III collagen, and increased the gene expression and protein level of α-SMA. TSA blocked the fibrogenic effects of TGF-β1. Conclusion.: The current results demonstrated the isolation of MSCs from LF. The cellular response to TGF-β1 implied that these cells might play an important role in the pathogenesis of LF hypertrophy. TSA, which blocks the effects of TGF-β1, may be a potent therapeutic choice for inhibiting LF hypertrophy.",
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AU - Lee, Hsieh Hsing

AU - Chiang, En Rung

AU - Lai, Hung Chang

AU - Chen, Ling Lan

AU - Lee, Yi Ting

AU - Tsai, Chih Chien

AU - Liu, Chien Lin

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