Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus

C. P. Gibbs, A. Tanaka, S. K. Anderson, J. Radul, J. Baar, A. Ridgway, H. J. Kung, D. J. Fujita

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalJournal of Virology
Volume53
Issue number1
Publication statusPublished - Mar 13 1985
Externally publishedYes

Fingerprint

src Genes
Rous sarcoma virus
Oncogenes
genes
loci
Alu Elements
Chickens
Restriction Mapping
restriction mapping
chickens
Introns
exons
introns
Exons
Pluto
Clone Cells
clones
repetitive sequences
Genomic Library
DNA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Gibbs, C. P., Tanaka, A., Anderson, S. K., Radul, J., Baar, J., Ridgway, A., ... Fujita, D. J. (1985). Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus. Journal of Virology, 53(1), 19-24.

Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus. / Gibbs, C. P.; Tanaka, A.; Anderson, S. K.; Radul, J.; Baar, J.; Ridgway, A.; Kung, H. J.; Fujita, D. J.

In: Journal of Virology, Vol. 53, No. 1, 13.03.1985, p. 19-24.

Research output: Contribution to journalArticle

Gibbs, CP, Tanaka, A, Anderson, SK, Radul, J, Baar, J, Ridgway, A, Kung, HJ & Fujita, DJ 1985, 'Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus', Journal of Virology, vol. 53, no. 1, pp. 19-24.
Gibbs CP, Tanaka A, Anderson SK, Radul J, Baar J, Ridgway A et al. Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus. Journal of Virology. 1985 Mar 13;53(1):19-24.
Gibbs, C. P. ; Tanaka, A. ; Anderson, S. K. ; Radul, J. ; Baar, J. ; Ridgway, A. ; Kung, H. J. ; Fujita, D. J. / Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus. In: Journal of Virology. 1985 ; Vol. 53, No. 1. pp. 19-24.
@article{87df0e8409ee481693bd89521a1068d2,
title = "Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus",
abstract = "We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.",
author = "Gibbs, {C. P.} and A. Tanaka and Anderson, {S. K.} and J. Radul and J. Baar and A. Ridgway and Kung, {H. J.} and Fujita, {D. J.}",
year = "1985",
month = "3",
day = "13",
language = "English",
volume = "53",
pages = "19--24",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus

AU - Gibbs, C. P.

AU - Tanaka, A.

AU - Anderson, S. K.

AU - Radul, J.

AU - Baar, J.

AU - Ridgway, A.

AU - Kung, H. J.

AU - Fujita, D. J.

PY - 1985/3/13

Y1 - 1985/3/13

N2 - We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.

AB - We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.

UR - http://www.scopus.com/inward/record.url?scp=0021969241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021969241&partnerID=8YFLogxK

M3 - Article

C2 - 2981336

AN - SCOPUS:0021969241

VL - 53

SP - 19

EP - 24

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -