Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus

C. P. Gibbs, A. Tanaka, S. K. Anderson, J. Radul, J. Baar, A. Ridgway, H. J. Kung, D. J. Fujita

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalJournal of Virology
Volume53
Issue number1
Publication statusPublished - Mar 13 1985
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Gibbs, C. P., Tanaka, A., Anderson, S. K., Radul, J., Baar, J., Ridgway, A., Kung, H. J., & Fujita, D. J. (1985). Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of rous sarcoma virus. Journal of Virology, 53(1), 19-24.