Isolation and structural elucidation by 2D NMR of planteose, a major oligosaccharide in the mucilage of chia (Salvia hispanica L.) seeds

Xiaohui Xing, Yves S.Y. Hsieh, Kuok Yap, Main E. Ang, Jelle Lahnstein, Matthew R. Tucker, Rachel A. Burton, Vincent Bulone

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An oligosaccharide was isolated in high purity and excellent yield from the water-extractable mucilage of chia (Salvia hispanica L.) seeds using an optimized solid-phase extraction method. LC–MS analysis showed that the compound presents a molecular mass of 504 Da and trifluoroacetic acid hydrolysis revealed that it consists of galactose, glucose and fructose. Glycosidic linkage analysis showed that the oligosaccharide contains two non-reducing ends corresponding to terminal glucopyranose and terminal galactopyranose, respectively. The oligosaccharide was identified as planteose by the complete assignment of a series of 2D NMR spectra (COSY, TOCSY, ROESY, HSQC, and HMBC). The significance of the presence of planteose in chia seeds is discussed in the context of nutrition and food applications.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalCarbohydrate Polymers
Volume175
DOIs
Publication statusPublished - Nov 1 2017
Externally publishedYes

Keywords

  • Chia
  • NMR
  • Planteose
  • Salvia hispanica L
  • Seed mucilage
  • Solid-phase extraction

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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