Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm

Pi Yu Chen, Yuh Chi Kuo, Chin Hui Chen, Yueh Hsiung Kuo, Ching Kuo Lee

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalana Perrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by 3H- thymidine uptake. (±)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4′-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC50 values 19.4, 73.8, and 58.8 μM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-γ in activated PBMC in a concentration-dependent manner.

Original languageEnglish
Pages (from-to)1789-1795
Number of pages7
JournalMolecules
Volume14
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Agave
Flavones
thymidine
Isoflavones
Spectroscopic analysis
Cell proliferation
spectroscopic analysis
Thymidine
leaves
Inhibitory Concentration 50
Interleukin-2
isolation
Cell Proliferation
cells
4-chromanone
dihydrobonducellin

Keywords

  • Agave sisalana Perrine ex Engelm
  • Homoisoflavonoid
  • IFN-γ
  • IL-2
  • PBMC

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm. / Chen, Pi Yu; Kuo, Yuh Chi; Chen, Chin Hui; Kuo, Yueh Hsiung; Lee, Ching Kuo.

In: Molecules, Vol. 14, No. 5, 05.2009, p. 1789-1795.

Research output: Contribution to journalArticle

Chen, Pi Yu ; Kuo, Yuh Chi ; Chen, Chin Hui ; Kuo, Yueh Hsiung ; Lee, Ching Kuo. / Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm. In: Molecules. 2009 ; Vol. 14, No. 5. pp. 1789-1795.
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