Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm

Pi Yu Chen, Yuh Chi Kuo, Chin Hui Chen, Yueh Hsiung Kuo, Ching Kuo Lee

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32 Citations (Scopus)


Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalana Perrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by 3H- thymidine uptake. (±)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4′-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC50 values 19.4, 73.8, and 58.8 μM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-γ in activated PBMC in a concentration-dependent manner.

Original languageEnglish
Pages (from-to)1789-1795
Number of pages7
Issue number5
Publication statusPublished - May 2009



  • Agave sisalana Perrine ex Engelm
  • Homoisoflavonoid
  • IFN-γ
  • IL-2
  • PBMC

ASJC Scopus subject areas

  • Organic Chemistry

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