Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice

Shin Ping Lin, Yin Hsuan Huang, Kai Di Hsu, Ying Jang Lai, Yu Kuo Chen, Kuan Chen Cheng

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17 Citations (Scopus)


A bacterial cellulose (BC) producing strain isolated from fermented fruit juice was identified as Komagataeibacter intermedius (K. intermedius) FST213-1 by 16s rDNA sequencing analysis and biochemical characteristics test. K. intermedius FST213-1 can produce BC within pH 4-9 and exhibit maximum BC production (1.2 g/L) at pH 8 in short-term (4-day) cultivation. Results of Fourier transform infrared spectroscopy, X-ray diffraction, water content, thermogravimetric analysis and mechanical property indicated that BC produced from K. intermedius FST213-1 exhibits higher water content ability (99.5%), lower thermostability (315 °C), lower crystallinity (79.3%) and similar mechanical properties in comparison with the specimen from model BC producer, Gluconacetobacter xylinus 23769. Based on these analyses, the novel based-resistant strain K. intermedius FST213-1 can efficiently produce BC, which can be applied for industrial manufacturing with potential features.

Original languageEnglish
Pages (from-to)827-833
Number of pages7
JournalCarbohydrate Polymers
Publication statusPublished - Oct 20 2016
Externally publishedYes



  • Bacterial cellulose
  • Base-resistant strain
  • Komagataeibacter intermedius
  • Materials property analysis
  • Strain selection

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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