Isolation and identification of a novel spotted fever group rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan

Kun Hsien Tsai, Hsi Chieh Wang, Chun Hsien Chen, Jyh Hsiung Huang, Hsiu Ying Lu, Chien Ling Su, Pei Yun Shu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel species of spotted fever group (SFG) rickettsia, Rickettsia spp. IG-1, was identified and isolated from adult Ixodes granulatus ticks collected from Orchid Island (Lanyu), an islet located in southeastern Taiwan. Serum samples collected from the rodent host Rattus losea and SFG-positive human serum reacted with IG-1 using an immunofluorescence assay (IFA). Pairwise nucleotide sequence analysis of 16S rRNA (rrs), gltA, ompA, ompB, and sca4 shows that IG-1 belongs to SFG rickettsiae and had the highest nucleotide sequence similarities to Rickettsia slovaca and R. sibirica. Phylogenetic analysis of the ompA, ompB genes and sca4 shows that IG-1 is most closely related to R. honei. The results showed the sequence divergence of this novel isolate from those rickettsiae previously characterized using the criteria proposed by Fournier and others. Whether IG-1 is pathogenic for humans remains to be studied.

Original languageEnglish
Pages (from-to)256-261
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number2
Publication statusPublished - Aug 1 2008
Externally publishedYes

Fingerprint

Rickettsia
Ixodes
Ticks
Taiwan
Islands
Fever
Serum
Fluorescent Antibody Technique
Sequence Analysis
Rodentia
Genes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Isolation and identification of a novel spotted fever group rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan. / Tsai, Kun Hsien; Wang, Hsi Chieh; Chen, Chun Hsien; Huang, Jyh Hsiung; Lu, Hsiu Ying; Su, Chien Ling; Shu, Pei Yun.

In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 2, 01.08.2008, p. 256-261.

Research output: Contribution to journalArticle

Tsai, Kun Hsien ; Wang, Hsi Chieh ; Chen, Chun Hsien ; Huang, Jyh Hsiung ; Lu, Hsiu Ying ; Su, Chien Ling ; Shu, Pei Yun. / Isolation and identification of a novel spotted fever group rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 79, No. 2. pp. 256-261.
@article{9a4bdcc497db45de94005c425880e90b,
title = "Isolation and identification of a novel spotted fever group rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan",
abstract = "A novel species of spotted fever group (SFG) rickettsia, Rickettsia spp. IG-1, was identified and isolated from adult Ixodes granulatus ticks collected from Orchid Island (Lanyu), an islet located in southeastern Taiwan. Serum samples collected from the rodent host Rattus losea and SFG-positive human serum reacted with IG-1 using an immunofluorescence assay (IFA). Pairwise nucleotide sequence analysis of 16S rRNA (rrs), gltA, ompA, ompB, and sca4 shows that IG-1 belongs to SFG rickettsiae and had the highest nucleotide sequence similarities to Rickettsia slovaca and R. sibirica. Phylogenetic analysis of the ompA, ompB genes and sca4 shows that IG-1 is most closely related to R. honei. The results showed the sequence divergence of this novel isolate from those rickettsiae previously characterized using the criteria proposed by Fournier and others. Whether IG-1 is pathogenic for humans remains to be studied.",
author = "Tsai, {Kun Hsien} and Wang, {Hsi Chieh} and Chen, {Chun Hsien} and Huang, {Jyh Hsiung} and Lu, {Hsiu Ying} and Su, {Chien Ling} and Shu, {Pei Yun}",
year = "2008",
month = "8",
day = "1",
language = "English",
volume = "79",
pages = "256--261",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "2",

}

TY - JOUR

T1 - Isolation and identification of a novel spotted fever group rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan

AU - Tsai, Kun Hsien

AU - Wang, Hsi Chieh

AU - Chen, Chun Hsien

AU - Huang, Jyh Hsiung

AU - Lu, Hsiu Ying

AU - Su, Chien Ling

AU - Shu, Pei Yun

PY - 2008/8/1

Y1 - 2008/8/1

N2 - A novel species of spotted fever group (SFG) rickettsia, Rickettsia spp. IG-1, was identified and isolated from adult Ixodes granulatus ticks collected from Orchid Island (Lanyu), an islet located in southeastern Taiwan. Serum samples collected from the rodent host Rattus losea and SFG-positive human serum reacted with IG-1 using an immunofluorescence assay (IFA). Pairwise nucleotide sequence analysis of 16S rRNA (rrs), gltA, ompA, ompB, and sca4 shows that IG-1 belongs to SFG rickettsiae and had the highest nucleotide sequence similarities to Rickettsia slovaca and R. sibirica. Phylogenetic analysis of the ompA, ompB genes and sca4 shows that IG-1 is most closely related to R. honei. The results showed the sequence divergence of this novel isolate from those rickettsiae previously characterized using the criteria proposed by Fournier and others. Whether IG-1 is pathogenic for humans remains to be studied.

AB - A novel species of spotted fever group (SFG) rickettsia, Rickettsia spp. IG-1, was identified and isolated from adult Ixodes granulatus ticks collected from Orchid Island (Lanyu), an islet located in southeastern Taiwan. Serum samples collected from the rodent host Rattus losea and SFG-positive human serum reacted with IG-1 using an immunofluorescence assay (IFA). Pairwise nucleotide sequence analysis of 16S rRNA (rrs), gltA, ompA, ompB, and sca4 shows that IG-1 belongs to SFG rickettsiae and had the highest nucleotide sequence similarities to Rickettsia slovaca and R. sibirica. Phylogenetic analysis of the ompA, ompB genes and sca4 shows that IG-1 is most closely related to R. honei. The results showed the sequence divergence of this novel isolate from those rickettsiae previously characterized using the criteria proposed by Fournier and others. Whether IG-1 is pathogenic for humans remains to be studied.

UR - http://www.scopus.com/inward/record.url?scp=49449106295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49449106295&partnerID=8YFLogxK

M3 - Article

C2 - 18689633

AN - SCOPUS:49449106295

VL - 79

SP - 256

EP - 261

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 2

ER -