Isolation and characterization of thioredoxin h cDNA from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots

Dong Jiann Huang, Hsien Jung Chen, Wen Chi Hou, Yaw Huei Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Three full-length cDNA clones, designated TRX1, TRX2, and TRX3 encoding different but similar thioredoxin h polypeptides, were isolated from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots. These three thioredoxin h clones were similar to each other and contained the canonical WCGPC active site and the important structural and functional amino acids that were conserved in thioredoxin sequences. Recombinant thioredoxin h (TRX2) overproduced in Escherichia coli (M15) was purified by Ni2+-chelated affinity chromatography. The molecular mass of TRX2 is ca. 1.4kDa determined by SDS-PAGE. TRX2 carries an N-terminal 17-amino acid extension enriched in hydrophobic residues. In Northern blot analysis, mRNAs corresponding to all three thioredoxin genes were found to have the highest level in the storage roots; those corresponding to TRX2 and TRX3 were detected at the next higher level in flowers. All three transcripts were detected at very low levels in sprouts of storage roots, roots, veins, and leaves. In Western blot analysis, the thioredoxins were found to have the highest level in the storage roots and veins; higher level in leaves; and very low levels in sprouts of storage root and roots. Active recombinant TRX2 protein is able to reduce the TI proteins in NTR system. Thioredoxin TRX2 was not reduced efficiently when NTR was removed from the NTR system. Three thioredoxin h genes of sweet potato storage roots display differential gene expression patterns, which may be associated with the diverse roles and functions they play in plant physiology in order to cope with particular developmental and environmental cues.

Original languageEnglish
Pages (from-to)515-523
Number of pages9
JournalPlant Science
Volume166
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Thioredoxin h
Ipomoea batatas
Thioredoxins
sweet potatoes
Complementary DNA
Veins
Clone Cells
Genes
Plant Physiological Phenomena
Affinity chromatography
Amino Acids
Recombinant proteins
Physiology
Molecular mass
plant veins
Affinity Chromatography
Recombinant Proteins
Gene expression
Northern Blotting
Escherichia coli

Keywords

  • cDNA sequence
  • Gene expression
  • Recombinant protein
  • Sweet potato
  • Thioredoxin h

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Isolation and characterization of thioredoxin h cDNA from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots. / Huang, Dong Jiann; Chen, Hsien Jung; Hou, Wen Chi; Lin, Yaw Huei.

In: Plant Science, Vol. 166, No. 2, 02.2004, p. 515-523.

Research output: Contribution to journalArticle

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abstract = "Three full-length cDNA clones, designated TRX1, TRX2, and TRX3 encoding different but similar thioredoxin h polypeptides, were isolated from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots. These three thioredoxin h clones were similar to each other and contained the canonical WCGPC active site and the important structural and functional amino acids that were conserved in thioredoxin sequences. Recombinant thioredoxin h (TRX2) overproduced in Escherichia coli (M15) was purified by Ni2+-chelated affinity chromatography. The molecular mass of TRX2 is ca. 1.4kDa determined by SDS-PAGE. TRX2 carries an N-terminal 17-amino acid extension enriched in hydrophobic residues. In Northern blot analysis, mRNAs corresponding to all three thioredoxin genes were found to have the highest level in the storage roots; those corresponding to TRX2 and TRX3 were detected at the next higher level in flowers. All three transcripts were detected at very low levels in sprouts of storage roots, roots, veins, and leaves. In Western blot analysis, the thioredoxins were found to have the highest level in the storage roots and veins; higher level in leaves; and very low levels in sprouts of storage root and roots. Active recombinant TRX2 protein is able to reduce the TI proteins in NTR system. Thioredoxin TRX2 was not reduced efficiently when NTR was removed from the NTR system. Three thioredoxin h genes of sweet potato storage roots display differential gene expression patterns, which may be associated with the diverse roles and functions they play in plant physiology in order to cope with particular developmental and environmental cues.",
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